Page 39 - 《中国药科大学学报》2025年第5期

第 56 卷第 5 期 黄 雍，等：黄芪显微组织化学定位研究与应用 571

发现叶中皂苷含量最高，茎中黄酮含量最高 ，但针 Chin Med Pharm(中医药导报), 2016, 22(24): 76-79.
[8]
对根内部不同组织（如木质部、韧皮部、周皮）间有 [5] Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia:
part 1(中华人民共和国药典: 一部)[S]. Beijing: China Medi-
效成分分布的研究较少。本研究分离不同部位，定 cal Science Press, 2020: 315.
量检测各部位的总皂苷、总黄酮及黄芪甲苷含量， [6] Ma YY, Wang J, Luo Q, et al. Pharmacological effects and re-
明确了总皂苷在韧皮部中含量最高，总黄酮在周皮 search progress of total astragalus saponins[J]. J Liaoning Coll
Tradit Chin Med(辽宁中医药大学学报), 2020, 22(7): 153-157.
中含量最高，并且周皮总黄酮含量可达韧皮部的 [7] He JJ, Qin C, He LQ, et al. Research on flavonoids of
2 倍以上，进一步验证了显微组织化学定位方法的 Huangqi(Astragali Radix)and their pharmacological action[J]. J
准确性。同时，本研究发现黄芪甲苷特异性富集于 Liaoning Coll Tradit Chin Med(辽宁中医药大学学报), 2024,
26(1): 112-119.
周皮，与相关文献研究相符 ，周皮中黄芪甲苷含量 [8] Teng HM, Wang LL, Hu XC, et al. Anatomy of vegetative or-
[14]
高达 0.850%。值得关注的是，尽管周皮在整根中占 gans and the accumulation of active constituents in Astragalus
比低 ，黄芪甲苷贡献率却接近 100%（表 1）。鉴于 membranaceus var. mongolicus[J]. Acta Bot Boreali- Occident
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Sin(西北植物学报), 2017, 37(2): 279-285.
黄芪甲苷是黄芪甲苷类皂苷在碱性条件下的转化
[9] Sun GZ, Qian M, Zhou Y, et al. Determination of flavonoids in
[16]
产物 ，各部位总皂苷含量均在 4% 以上，由此推测 seabuckthorn leaves of Gansu[J]. China Food Ind(中国食品工
韧皮部和木质部可能特异性富集其他类皂苷。 业), 2019, 5: 60-64.
[10] Zhou L, Liu YH. Drug Inspection Technology(药物检验技
本研究建立的亚硝酸钠-硝酸铝-氢氧化钠染色
术)[M]. Chengdu: Southwest Jiaotong University Press, 2016:
法结合水合氯醛透化技术，突破了传统氢氧化钠染 28.
色法受中药材自身颜色干扰的局限，为黄芪及其他 [11] Zhu XY, Yang WZ, Yang SB, et al. Tissue structure and histo-
chemistry localization from different vegetative organs of
中药材有效成分的可视化分析提供参考，未来可进
Dactylicapnos scandens[J]. Southwest China J Agric Sci(西南
一步应用于黄芪品种鉴定、产地质量评价及次生代 农业学报), 2022, 35(2): 304-309.
谢产物合成通路解析，为定向培育高活性成分含量 [12] Wang J. Developmental anatomy of medicinal parts of Sophora
的黄芪品种及开发高效提取工艺提供细胞学层面 flavescens as well as age identification and study of accumula-
tion of secondary metabolite of astragalus(苦参药用部位发育
的理论依据。 解剖暨黄芪道地药材的年限鉴别及其次生代谢产物积累研
究)[D]. Hefei: Anhui University of Chinese Medicine, 2013.
[13] Zhu XB, Zhang GF, Chen P. Research progress in the transcrip-
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