Page 44 - 《中国药科大学学报》2026年第2期

170 学报 Journal of China Pharmaceutical University 2026, 57(2): 163 − 171 第 57 卷

官或动物模型开展转录组学与代谢组学分析，构建 人胰脂肪酶抑制剂的发现及构效关系研究)[D]. Shihezi: Shi-
“成分—靶点—通路—表型”网络，从系统层面阐释 hezi University, 2022.
[14] Hou FB, Zhang N, Zhu GH, et al. Functional imaging and in-
其多靶点协同机制，为基于中药及天然产物的胰脂 hibitor screening of human pancreatic lipase by a resorufin-
肪酶抑制剂开发提供新思路与新方法。 based fluorescent probe[J]. Biosensors (Basel), 2023, 13(2):
283.
[15] Maghraby YR, El-Shabasy RM, Ibrahim AH, et al. Enzyme im-
References
mobilization technologies and industrial applications[J]. ACS
[1] Zhang YH, Yao YJ. The association between obesity indicators Omega, 2023, 8(6): 5184-5196.
and mortality among individuals with hyperlipidemia: evidence [16] Guimarães JR, Oliveira KSGC, Gonçalves MCP, et al. A re-
from the NHANES 2003-2018[J]. Lipids Health Dis, 2025, view of lipase immobilization on hydrophobic supports incorpo-
24(1): 20. rating systematic mapping principles[J]. React Chem Eng, 2023,
[2] Subramaniyan V, Hanim YU. Role of pancreatic lipase inhibi- 8(11): 2689-2702.
tion in obesity treatment: mechanisms and challenges towards [17] Zeng F, Wu WX, Zhang YY, et al. Rapid screening of lipase in-
current insights and future directions[J]. Int J Obes (Lond), hibitors in licorice extract by using porcine pancreatic lipase im-
2025, 49(3): 492-506. mobilized on Fe 3 O 4 magnetic nanoparticles[J]. Food Funct,
[3] Gudzune KA, Kushner RF. Medications for obesity: a 2021, 12(12): 5650-5657.
review[J]. JAMA, 2024, 332(7): 571-584. [18] Amini Y, Shahedi M, Habibi Z, et al. A multi-component reac-
[4] Zhang R, Xing LL, Wang X, et al. Inhibition of pancreatic li- tion for covalent immobilization of lipases on amine-functional-
pase and cholesterol by hawthorn extract: a study of binding ized magnetic nanoparticles: production of biodiesel from waste
mechanisms and inhibitor screening[J]. Int J Biol Macromol, cooking oil[J]. Bioresour Bioprocess, 2022, 9(1): 60.
2025, 311(Pt2): 143680. [19] Xu JF, Cao PK, Fan ZY, et al. Rapid screening of lipase in-
[5] Wang ZY, Ren XW, Peng Z, et al. Flavonoid-rich extracts of hibitors in Scutellaria baicalensis by using porcine pancreatic
Nelumbo nucifera leaves alleviate obesity in HFD-fed mice via lipase immobilized on magnetic core-shell metal-organic frame-
microbiota-dependent modulation of brown fat works[J]. Molecules, 2022, 27(11): 3475.
thermogenesis[J]. J Ethnopharmacol, 2026, 354: 120513. [20] Zhu YT, Ren XY, Yuan L, et al. Fast identification of lipase in-
[6] Wu LJ, Wang XJ, Jiang JH, et al. Mechanism of rhubarb in the hibitors in oolong tea by using lipase functionalised Fe 3 O 4 mag-
treatment of hyperlipidemia: a recent review[J]. Open Med netic nanoparticles coupled with UPLC-MS/MS[J]. Food Chem,
(Wars), 2023, 18(1): 20230812. 2015, 173: 521-526.
[7] Dong XY, Wen YX, Nie Q, et al. Effects of octacosanol isolat- [21] Chen XL, Xue S, Lin YL, et al. Immobilization of porcine pan-
ed from Moringa oleifera leaves on inhibiting the activity of creatic lipase onto a metal-organic framework, PPL@MOF: a
pancreatic lipase[J]. J Agric Food Chem, 2025, 73(12): 7202- new platform for efficient ligand discovery from natural
7210. herbs[J]. Anal Chim Acta, 2020, 1099: 94-102.
[8] Jin PY, Chen LZ, Zhong JJ, et al. Screening and identification [22] Tadesse M, Liu Y. Recent advances in enzyme immobilization:
of lipase inhibitors extracted from Dioscorea nipponica makino the role of artificial intelligence, novel nanomaterials, and dy-
by UV-vis and HPLC coupled to UPLC-Q-TOF-MS/MS[J]. Int namic carrier systems[J]. Catalysts, 2025, 15(6): 571.
J Biol Macromol, 2023, 230: 123427. [23] Fan WX. Inhibitor screening and inhibition of immobilized pan-
[9] Birari RB, Gupta S, Mohan CG, et al. Antiobesity and lipid creatic lipase(固定化胰脂肪酶的抑制剂筛选及抑制作用研
lowering effects of Glycyrrhiza chalcones: experimental and 究)[D]Dalian: Dalian University of Technology, 2024.
computational studies[J]. Phytomedicine, 2011, 18(8/9): 795- [24] Zhang H, Chen GL, Yang JP, et al. Screening and characterisa-
801. tion of potential antioxidant, hypoglycemic and hypolipidemic
[10] Jiang YY. Screening of lipase inhibitors in artemisia argyi components revealed in Portulaca oleracea via multi-target
leaves and preparation and release properties of rice starch in- affinity ultrafiltration LC-MS and molecular docking[J]. Phy-
clusion complex [D]. Chongqing: Chongqing Technology and tochem Anal, 2022, 33(2): 272-285.
Business University, 2022. [25] He YQ, Zhao XY, Yu MZ, et al. Affinity ultrafiltration mass
[11] Rocha S, Lobo MC, Proença C, et al. Optimization and imple- spectrometry for screening active ingredients in traditional Chi-
mentation of spectrophotometric and fluorimetric methodolo- nese medicine: a review of the past decade (2014-2024)[J].
gies to evaluate pancreatic lipase inhibitory using chalcones: a Molecules, 2025, 30(3): 608.
comparative study[J]. J Anal Test, 2025, 9(3): 334-345. [26] Huang H, Han MH, Gu Q, et al. Identification of pancreatic li-
[12] You Q, Chen F, Wang X, et al. Anti-diabetic activities of phe- pase inhibitors from Eucommia ulmoides tea by affinity-ultrafil-
nolic compounds in muscadine against alpha-glucosidase and tration combined UPLC-Orbitrap MS and in vitro validation[J].
pancreatic lipase[J]. LWT Food Sci Technol, 2012, 46(1): 164- Food Chem, 2023, 426: 136630.
168. [27] Quan SH, Wen MY, Xu P, et al. Efficient screening of pancreat-
[13] Qin XY. Discovery and structure-activity relationship of ic lipase inhibitors from Rheum palmatum by affinity ultrafiltra-
flavonoids inhibitors against human pancreatic lipase(黄酮类 tion-high-performance liquid chromatography combined with