Page 110 - 《中国药科大学学报》2026年第1期

104 学报 Journal of China Pharmaceutical University 2026, 57(1): 98 − 107 第 57 卷
A B C D E

4 3 ** 30 ** 90 ** 80 40 **
Neurological scores 2 1 Cerebral infarct rate/% 20 Brain water content/% 80 SOD/(U/mg protein) 60 ** MDA/(nmol/mg protein) 30

20
40
10
20
10
70
0 0 70 0 0 0
Sham Model HGWD-H Sham Model HGWD-H Sham Model HGWD-H Sham Model HGWD-H Sham Model HGWD-H

F G H I
15 ** 150 ** 200 ** Sham
NO/(mmol/mg protein) 10 5 TNF-α/(pg/mg protein) 100 IL-1β/(pg/mg protein) 150 Model

100
50
50

0 0 0 HGWD-H
Sham Model HGWD-H Sham Model HGWD-H Sham Model HGWD-H

-/-
Figure 3 Effect of HGWD on cerebral ischemia-reperfusion injury in Nrf2 mice( x ± s, n = 8)
A: Statistical results of Longa neurological score; B: Cerebral infarct rate (%) assessed by TTC staining calculated by TTC staining; C: Effect of
HGWD on brain water content; D-F: Effects of HGWD on the levels of SOD, MDA, and NO in brain tissue; G-H: Effect of HGWD on the levels of
TNF-α and IL-1β in brain tissue; I: Representative images of TTC-stained brain sections from each group
**P < 0.01 vs sham group
A 1.5 B 1.5 C 3

(FC) (FC) (FC) **
expression 1.0 expression 1.0 expression 2

mRNA 0.5 ** mRNA 0.5 ** mRNA 1

HO-1 0 Bcl-2 0 Bax 0
Sham Model HGWD-H Sham Model HGWD-H Sham Model HGWD-H

D E
Sham Model HGWD-H (FC) 2.0 HO-1 *
HO-1 32 kD Bcl-2
Bax
β-actin 42 kD expression 1.5
Bcl-2 26 kD 1.0
protein *
β-actin 42 kD
Bax 21 kD 0.5 **
Relative
β-actin 42 kD 0 Sham Model HGWD-H

-/-
Figure 4 Effects of HGWD on mRNA and protein expression of Nrf2/HO-1 and Bcl-2/Bax pathway in the brain of Nrf2 mice
( x ± s, n = 8)
-/-
-/-
A-C: mRNA expression levels of HO-1, Bcl-2 and Bax in the brain of Nrf2 - mice; D: Expression of HO-1, Bcl-2 and Bax in Nrf2 mice brain
assayed by Western blot; E:Quantification of HO-1, Bcl-2 and Bax protein levels
**P < 0.01 vs sham group