Page 99 - 《中国药科大学学报》2025年第4期
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第 56 卷第 4 期 李莹莹，等：丙酸钠缓解鼠柠檬酸杆菌诱导的小鼠结肠炎效果优于混合短链脂肪酸 495
A 10 5 Q1 1.47 10 5 Q1 2.70 10 5 Q1 2.24 5 PBS;
Q2
Q2
Q2
7.72
4.79
5.22
APC-H :: CD183 10 4 APC-H :: CD183 10 4 APC-H :: CD183 10 4 4 Pro;
10 3
10 3
10 3
SCFAs
10 2
10 2
10 2
10 1 Q4
Q3
Q3
Q3
62.1
66.4
59.0 34.7 10 1 Q4 30.0 10 1 Q4 23.7 3 #
10 0 10 0 10 0
10 0 10 1 10 2 10 3 10 4 10 5 10 6 10 0 10 1 10 2 10 3 10 4 10 5 10 6 10 0 10 1 10 2 10 3 10 4 10 5 10 6 Th1(% of lymphocyte)
FITC-H :: CD4 FITC-H :: CD4 FITC-H :: CD4 *
Cont Pro SCFAs 2
Q2
Q2
Q2
Q1 1.91 10 5 Q1 2.78 10 5 Q1 2.34 1
10 5
2.52
3.00
2.65
CD183 APC-H :: CD183 10 4 APC-H :: CD183 10 4 APC-H :: CD183 10 4 0
10 3
10 3
10 3
10 2
10 2
10 2
Q3
Q3
Q3
10 1 Q4
73.4
66.6 26.8 10 1 Q4 25.8 10 1 Q4 21.2 Cont C.r.
68.9
10 0 10 0 10 0
10 0 10 1 10 2 10 3 10 4 10 5 10 6 10 0 10 1 10 2 10 3 10 4 10 5 10 6 10 0 10 1 10 2 10 3 10 4 10 5 10 6
FITC-H :: CD4 FITC-H :: CD4 FITC-H :: CD4
C.r. C.r.+Pro C.r.+SCFAs
CD4
B 10 5 Q1 0.17 10 5 Q1 0.18 10 5 Q1 0.15
Q2
Q2
Q2
Comp-PE-A :: CD194 10 4 Comp-PE-A :: CD194 10 4 Comp-PE-A :: CD194 10 4 0.4 PBS;
0.09
0.11
0.17
10 3
10 3
10 3
Pro;
SCFAs
10 2
10 2
10 2
10 1
10 1
10 1
Q4
Q4
Q4
Q3
Q3
10 0 59.0
40.7
10 0 10 1 10 2 10 3 10 4 10 5 10 6
10 0 10 1 10 2 10 3 10 4 10 5 10 6 Q3 10 0 64.1 35.6 10 0 66.2 33.5
10 0 10 1 10 2 10 3 10 4 10 5 10 6
FITC-H :: CD4 FITC-H :: CD4 FITC-H :: CD4 Th2(% of lymphocyte) 0.2
Cont Q2 10 5 Q1 Pro Q2 10 5 Q1 SCFAs Q2
10 5 Q1
CD194 Comp-PE-A :: CD194 10 4 0.12 0.15 Comp-PE-A :: CD194 10 4 0.18 0.12 Comp-PE-A :: CD194 10 4 0.20 0.17 0
10 3
10 3
10 3
10 2
10 2
10 2
10 1
Q4
Q3
Q4
Q4
Q3
72.5
33.3
10 0
31.7
27.1
66.4
68.0
10 0
10 0
10 0 10 1 10 2 10 3 10 4 10 5 10 6 Q3 10 1 10 0 10 1 10 2 10 3 10 4 10 5 10 6 10 1 10 0 10 1 10 2 10 3 10 4 10 5 10 6 Cont C.r.
FITC-H :: CD4 FITC-H :: CD4 FITC-H :: CD4
C.r. C.r.+Pro C.r.+SCFAs
CD4
C 10 4 21.7 1.62 10 4 35.3 2.17 10 4 32.0 2.20
Q1
Q2
Q2
Q2
PE-Cy7-H :: CD196 10 5 PE-Cy7-H :: CD196 10 5 PE-Cy7-H :: CD196 10 5 4 PBS;
Q1
Q1
10 3
10 3
10 3
Pro;
10 2
10 2
10 2
SCFAs
Q3
Q3
10 1 Q4
Q3
$
35.5
33.3
10 0
10 0
10 0 38.2 38.4 10 1 Q4 29.3 10 1 Q4 30.4 3 ##
10 0 10 1 10 2 10 3 10 4 10 5 10 0 10 1 10 2 10 3 10 4 10 5 10 0 10 1 10 2 10 3 10 4 10 5
FITC-H :: CD4 FITC-H :: CD4 FITC-H :: CD4 *
Cont Q2 Q1 Pro Q2 Q1 SCFAs Q2 Th17(% of lymphocyte) 2
31.9
CD196 PE-Cy7-H :: CD196 10 5 Q1 2.07 PE-Cy7-H :: CD196 10 5 39.7 2.58 PE-Cy7-H :: CD196 10 5 43.5 2.12 1
10 4
10 4
10 4
10 3
10 3
10 3
10 2
10 2
10 2
Q3
Q3
10 1 Q4
Q3
30.4
28.4
10 0
10 0 31.1 34.9 10 1 Q4 29.3 10 1 Q4 23.7 0 Cont C.r.
10 0
10 0 10 1 10 2 10 3 10 4 10 5 10 0 10 1 10 2 10 3 10 4 10 5 10 0 10 1 10 2 10 3 10 4 10 5
FITC-H :: CD4 FITC-H :: CD4 FITC-H :: CD4
C.r. C.r.+Pro C.r.+SCFAs
CD4
D 10 5 Q1 2.22 10 5 Q1 2.95 10 5 Q1 2.50
PerCP-Cy5-5-H :: CD25 10 3 Q3 PerCP-Cy5-5-H :: CD25 10 3 Q3 PerCP-Cy5-5-H :: CD25 10 3 Q3 4 PBS;
Q2
Q2
Q2
0.52
0.98
0.37
10 4
10 4
10 4
Pro;
10 2
10 2
10 2
SCFAs
10 1 Q4
10 1 Q4
10 1 Q4
$
30.0
35.5
67.0
30.9
61.3
65.8
10 0
10 0 10 1 10 2 10 3 10 4 10 5 10 0 10 0 10 1 10 2 10 3 10 4 10 5 10 0 10 0 10 1 10 2 10 3 10 4 10 5 3 ##
FITC-H :: CD4 FITC-H :: CD4 FITC-H :: CD4
Cont 1.79 10 5 Q1 Pro 2.35 10 5 Q1 SCFAs 2.09 Treg(% of lymphocyte) 2 *
PerCP-Cy5-5-H :: CD25 10 2 Q3 PerCP-Cy5-5-H :: CD25 10 2 Q3 PerCP-Cy5-5-H :: CD25 10 2 Q3
10 5 Q1
Q2
Q2
Q2
CD25 10 4 0.39 10 4 0.40 10 4 0.82 1
10 3
10 3
10 3
10 1 Q4
74.1
65.0
37.0
60.8
10 0
10 0
10 0
10 0 10 1 10 2 10 3 10 4 10 5
10 0 10 1 10 2 10 3 10 4 10 5
10 0 10 1 10 2 10 3 10 4 10 5 10 1 Q4 32.2 10 1 Q4 23.0 0 Cont C.r.
FITC-H :: CD4 FITC-H :: CD4 FITC-H :: CD4
C.r. C.r.+Pro C.r.+SCFAs
CD4
+
Figure 5 Sodium propionate can affects CD4 Th cell differentiation compared with SCFAs ( x±SE, n=3-5)
+
+
+
+
+
+
+
+
A-D: The differentiation of CD4 CD183 (Th1), CD4 CD194 (Th2), CD4 CD196 (Th17) and CD4 CD25 (Treg) cells in mesenteric lymph
nodes (MLNs) was detected by flow cytometry
#
$
##
* P<0.05 vs Cont group; P<0.05, P<0.01 vs C.r.+PBS group; P<0.05 vs C.r.+SCFAs group
