Page 96 - 《中国药科大学学报》2025年第4期

492 学报 Journal of China Pharmaceutical University 2025, 56(4): 488 − 497 第 56 卷
A 1.5 pH 4.8 B 1.1 pH 7.0 C 0.8 pH 7.5

0;
P-8;
P-25; &&& ***
###
A-13.5; *** &&&
###
1.0 A-42.187 5; &&&
B-5.18;
###
A 600 nm B-16.187 5; ### *** A 600 nm 0.5 & ### A 600 nm 0.4
###
S-26.68;
&&&
S-83.375
###
0.5 &&&
&&&
###
&&&
0 0 0
0 2 4 6 8 0 2 4 6 8 0 2 4 6 8
p/h p/h p/h
D 1.5 8 mmol/L ** E 1.5 25 mmol/L
mRNA expression 1.0 ** ** mRNA expression 1.0
0.5
0.5
*** ***
***
*** *** *** ***
0 0
Cont NleA NleB NleH EspI EspZ Tir Cont NleA NleB NleH EspI EspZ Tir
Figure 1 Sodium propionate significantly inhibited the growth of Citrobacter rodentium (C.r.) compared with mixed SCFAs ( x±SE, n=3-4)
A-C: Under different pH conditions, different components of SCFAs were co-cultured with C.r. in vitro, and the changes of C.r. growth
&
***
were monitored. P<0.001 vs 0 mmol/L group; ### P<0.001 vs P-8 mmol/L group; P<0.05, &&& P<0.001 vs P-25 mmol/L group; D-E:
Different concentrations of sodium propionate were co-cultured with C.r. to detect the expression of colonization virulence factors.
**P<0.01, ***P<0.001 vs Cont group
A 110 B 10 11

C.r.;
C.r.+Pro;
10 10 9 C.r.+SCFAs ***
Body weight change/% 100 @@ * @@@ ** @@ @@ @@@ Bacterial output/g feces 10 8 7 *** * *** ** *** *
&&
&
&
#
#
#
10
##
#
***
***
10
Cont;
90
C.r.+Pro;
Pro;
C.r.+SCFAs
SCFAs; C.r.; 10 6
10 5
0 1 3 5 7 9 11 13 0 1 3 5 7 9 11 13
Day post C.r. infection Day post C.r. infection
Figure 2 Sodium propionate significantly reduces the degree of weight loss and intestinal bacterial output in infected mice compared
with mixed SCFAs ( x±SE, n=5-6)
A: Sodium Propionate and SCFAs were intervention in C.r. infected mice, and the weight changes were monitored during modeling,
* P<0.05, ** P<0.01, *** P<0.001 C.r. vs Cont; P<0.05 C.r.+Pro vs Pro; @@ P<0.01, @@@ P<0.001 C.r.+SCFAs vs SCFAs; P<0.05,
&
#
&& P<0.01 C.r.+Pro vs C.r.; B: Detection of intestinal bacterial output of mice in each infection group, * P<0.05, ** P<0.01, *** P<0.001 vs
#
##
C.r.; P<0.05, P<0.01 C.r.+Pro vs C.r.+SCFAs
22）可促进 Reg3γ 生成，IL-22 还与肠道白介素- 肠上皮机械屏障功能的改变。结果表明，C.r.诱导
17（IL-17）共同促进肠上皮细胞抵御感染。研究 肠通透性增加，而丙酸钠和混合 SCFAs 可降低这种
发现，C.r.+Pro 组 IL-17、IL-22 和 Reg3γ 水平高于 损伤。且相比较混合 SCFAs，丙酸钠减少损伤的效
C.r.组 （ 图 4-A−4-C） ， 且 C.r.+Pro 组 IL-17 和 果更好（图 4-D）。

Reg3γ 水平高于 C.r.+SCFAs 组。除了黏液屏障，肠 3.5 丙酸钠较 SCFAs 明显影响 CD4 Th 细胞分化
+
上皮细胞紧密连接形成的机械屏障也发挥重要的 在感染的过程中，肠道主要发挥适应性免疫反
抗感染作用。因此，灌胃小鼠 FITC-葡聚糖检测了 应以抵抗感染。CD4 T + 细胞是肠道适应性免疫应