Page 107 - 《渔业研究》2025年第5期

648 渔 业 研 究 第 47 卷

灿烂弧菌、溶藻弧菌具有不同的关键结构域导致的。 [J]. Aquaculture, 2020, 519: 734897.
抗生素曾经在弧菌病的防治中发挥重要作用， ［ 4 ］ Gómez-León J, Villamil L, Lemos M L, et al. Isolation
但是抗生素在环境中的残留亦可导致病原菌抗性基 of Vibrio alginolyticus and Vibrio splendidus from aqua-
因的产生、传播及环境微生态的破坏 [34] 。与抗生素 cultured carpet shell clam (Ruditapes decussatus) larvae
相比， “抗毒力”策略靶向病原菌的致病因子与致 associated with mass mortalities[J]. Applied and Envir-
病过程，在病害防控中发挥更为积极的作用 [35] 。 onmental Microbiology, 2005, 71(1): 98 − 104.
本研究在明确大牡蛎弧菌 S2 胶原酶作为致病因子 ［ 5 ］ Travers M A, Miller K B, Roque A, et al. Bacterial dis-
及其对菲律宾蛤仔免疫调控基础上，以大牡蛎弧 eases in marine bivalves[J]. Journal of Invertebrate Patho-
菌 S2 胶原酶活性为抑制靶标，从蛋白酶抑制剂中 logy, 2015, 131: 11 − 31.
［ 6 ］ Garnier M, Labreuche Y, Garcia C, et al. Evidence for
筛选了有效药物，即苯扎氯氨、盐酸巴马汀、2’-
the involvement of pathogenic bacteria in summer mor-
羟基查尔酮、氯己定、核黄素和原花青素。这些药
talities of the Pacific oyster Crassostrea gigas[J]. Mi-
物均可与大牡蛎弧菌 S2 胶原酶结合，进而对胶原
crobial Ecology, 2007, 53(2): 187 − 196.
酶活性产生抑制作用。这是首次针对水产养殖动物
［ 7 ］ Guillard R R L. Further evidence of the destruction of bi-
胶原酶活性为指标的生物制剂筛选，为后续弧菌病
valve larvae by bacteria[J]. The Biological Bulletin, 1959,
的防控提供新思路。
117(2): 258 − 266.
本研究克隆了大牡蛎弧菌 S2 胶原酶，并在大
［ 8 ］ Kesarcodi-Watson A, Kaspar H, Lategan M J, et al.
肠杆菌表达菌株中重组表达，获得了 rCol 。rCol Vc
Vc
Challenge of New Zealand Greenshell™ mussel Perna
可降解菲律宾蛤仔的胶原蛋白，且注射 rCol V c 可导
canaliculus larvae using two Vibrio pathogens: a hatch-
致菲律宾蛤仔个体存活率下降，因此胶原酶是大牡
ery study[J]. Diseases of Aquatic Organisms, 2009, 86
蛎弧菌 S2 的致病因子。注射胶原酶后，菲律宾蛤
(1): 15 − 20.
仔体内下调的差异表达基因显著富集于 DNA 重
［ 9 ］ Rahmani A, Delavat F, Lambert C, et al. Implication of
组、DNA 整合、DNA 代谢等生物学过程，以及肌
the type Ⅳ secretion system in the pathogenicity of Vi-
动蛋白结合、细胞骨架蛋白结合等分子结合功能；
brio tapetis, the etiological agent of brown ring disease
下调的差异表达基因主要富集于肌动蛋白细胞骨架 affecting the Manila clam Ruditapes philippinarum[J].
调控通路。这些发现为进一步理解弧菌的致病机制 Frontiers in Cellular and Infection Microbiology, 2021,
提供了重要的理论基础，同时也为菲律宾蛤仔弧菌
11: 634427.
病的防控提供了潜在的靶位点。 ［10］ Liu Y, Liao K, Zhu Y, et al. Effects of dietary taurine on
growth, taurine metabolism and Vibrio crassostreae res-
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