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1 期 雷锡俊，等：硒代蛋氨酸对黄颡鱼生长性能、饲料利用、肌肉组织学和抗氧化性能的影响 50 卷

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the National Academy of Sciences of the United States of oxidative stress-related parameters in rainbow trout juveniles

America, 2004, 101(7): 2046-2051. under chronic stress exposure[J]. Aquaculture, 2020, 529:
[29] Fontagné-Dicharry S, Véron V, Larroquet L, et al. Effect of sel- 735684.

Effects of selenomethionine on the growth performance, feed utilization,
muscle histology and antioxidant responses of yellow catfish
(Pelteobagrus fulvidraco)

LEI Xijun , KE Jiang , YANG Hong , XU Pengcheng , ZHANG Xu ,
HAO Zhiwei , LUO Zhi *
(College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China)

Abstract: To investigate the effects of selenomethionine on growth performance and muscle tissue of Pelteobagrus fulvidraco,
we formulated six experimental diets with selenomethionine as the Se source. The supplemental levels of selenomethionine in
the six experimental diets (S0, S1, S2, S3, S4, S5) were 0, 0.15, 0.30, 0.45, 0.60 and 0.75 mg/kg, respectively. The Se contents
in diets were 0.17, 0.22, 0.29, 0.33, 0.41 and 0.45 mg/kg, respectively. The feeding experiment lasted for 10 weeks. The P.
fulvidraco fed with S1 group feed showed the best growth performance and feed utilization rate, and had the most intact muscle
histology. However, with the increase of selenomethionine levels in S1-S5 groups, the growth performance and feed utilization
rate of P. fulvidraco decreased, the structural integrity of muscle tissue decreased, and the density and diameter of muscle fiber
decreased and shortened, indicating that muscle tissue had been damaged. In addition, with the increase of dietary selenometine
levels, the selenium content and the activities of antioxidant enzymes CAT, T-SOD and T-AOC in muscle tissue were
increased. The mRNA expression of antioxidation-related genes (cat, sod1, sod2 and nrf2) and selenproteins (txnrd, sps2, gpx1,
gpx2, msrb1, selenok and selenow) was significantly up-regulated, and the protein expression of NRF2 was significantly up-reg-
ulated. These results indicated that the antioxidant capacity and the expression level of selenoproteins in muscle of P. fulvidraco
increased with the increase of dietary selenomethionine levels. Dietary supplementation of excessive selenomethionine can
affect the growth performance of P. fulvidraco, reduce feed utilization, cause muscle tissue damage and reduce antioxidant
capacity. This study determined that adding 0.15 mg/kg of selenomethionine to the diets of P. fulvidraco is most beneficial for
its growth, and found that appropriate addition of selenomethionine can enhance the antioxidant capacity of P. fulvidraco
muscles.
Key words: Pelteobagrus fulvidraco; selenomethionine; growth performance; muscle; selenoprotein; antioxidant response
Corresponding author: LUO Zhi. E-mail: luozhi99@mail.hzau.edu.cn

Funding projects: National Natural Science Foundation of China (32530109); National Key Research and Development Pro-
gram of China (2023YFD2400600)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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