Page 151 - 《水产学报》2025年第5期

边延欣，等 水产学报, 2025, 49(5): 059613

表 2 引物序列
Tab. 2 Primer sequences
基因 上游引物 下游引物 登录号
gene forward sequence (5′–3′) reverse sequence (5′–3′) GenBank no.
il-8 CGTTGAACAGACTGGGAGAGATG AGTGGGATGGCTTCATTATCTTGT XM_038713528.1
il-10 CGGCACAGAAATCCCAGAGC CAGCAGGCTCACAAAATAAACATCT XM_038696252.1
tnf-α CTTCGTCTACAGCCAGGCATCG TTTGGCACACCGACCTCACC XM_038710731.1
tgf-β GCTCAAAGAGAGCGAGGATG TCCTCTACCATTCGCAATCC XM_038693206.1
nf-κb AGAAGACGACTCGGGGATGA GCTTCTGCAGGTTCTGGTCT XM_038699793.1
sod CCACCAGAGGTCTCACAGCA CCACTGAACCGAAGAAGGACT XM_038713339.1
cat TGGTGTTCACGGATGAGATGG GGAGAAGCGGACAGCAATAGG XM_038704976.1
pepck TGCGTGGATGAAGTTCGACA GGGGTTGGTCTTGTCTGAGG XM_038696647.1
g6pase GCCTACTGCGTAACATGGGT ATCCTGCCTTGAAACTGGCA XM_038735544.1
pfk GTGGACGGTGGAGACAACAT GTGATACTGCGCTGCACAAG XM_038724196.1
pk CTCTTTCATCCGCAAAGC AATTCCCAGGTCACCACG XM_038700627.1
glut2 GAGCCCACGGTACCTTTACA ACAGAGGAGCGGATCAAAGA XM_038728861.1
gk GGGGATGGAAAGCAAATCTACAAT CACACATACGAGCAGAGCGAGT XM_038703173.1
gs TCACAGCCATTGAGGCAGAG GTAAAAGTGCCCCCTGACGA XM_038697432.1
fbpase CTTCACCTCCTGTGTGCTTG CAGCTGGCTCACCATCTGTA XM_038733827.1
acc1α ATCCCTCTTTGCCACTGTTG GAGGTGATGTTGCTCGCATA XM_038709737.1
fasn TGTGGTGCTGAACTCTCTGG CATGCCTAGTGGGGAGTTGT XM_038735140.1
atgl CCATGATGCTCCCCTACACT GGCAGATACACTTCGGGAAA XM_038705351.1
hsl ATCAGAGCTGGAGCACCCTA GCAGAGGAGAGCAGAAAGGA XM_038710965.1
mgl AAGGTTTTTCTGGCGAAGGT CGTGGAAGTTCAGCTCATCA XM_038723003.1
pparα CCACCGCAATGGTCGATATG TGCTGTTGATGGACTGGGAAA XM_038705497.1
srebf1 TTCCTCTCCCTCAACCCTC AACCCCAGAAACCAGAATACC XM_038699585.1
lxr-1 CTCCCACCCCAATGACTTCC GCAGGCCCCTTCTTTCTCTT XM_038700506.1
lpl AACCGCAATCCCTCGCC AAGGTCTGTGTTTCTGAGTTGA XM_038705351.1
gpat4 GTGTAAAGGCAGCCATAGCC GTGCCCACCAGCATTTTACT XM_038704699.1
dgat1 CACGCCTCTTCTTGGAGAAC AATGGTACCCACAGCCAGAC XM_038724648.1
acadm TGGCTGAGATGGCAATGAAGG TTGGCGATGGAGGCGTAGTA XM_038717481.1
β-actin AAAGGGAAATCGTGCGTGAC AAGGAAGGCTGGAAGAGGG XM_038695351.1
注：il-8. 白细胞介素-8，il-10. 白细胞介素-10，tnf-α. 肿瘤坏死因子α，tgf-β. 转化生长因子β，nf-κb. 核因子 kappa B，cat. 过氧化氢酶，sod. 超
氧化物歧化酶，pepck. 磷酸烯醇式丙酮酸羧化激酶，G6pase. 葡萄糖-6-磷酸酶，pfk. 磷酸果糖激酶，pk. 丙酮酸激酶，gk. 葡萄糖激酶，gs. 糖原
合成酶，glut2. 葡萄糖转运蛋白-2，fbpase. 果糖-1, 6-二磷酸酶，acc1α. 乙酰辅酶A羧化酶， fasn. 脂肪酸合成酶，atgl. 脂肪甘油三酯脂酶，hsl.
激素敏感脂肪酶，mgl. 单酰甘油脂肪酶，pparα. 过氧化物酶体增殖物激活受体α，srebp1. 固醇调控元件结合蛋白1，lxr-1. 肝X受体，lpl. 脂蛋
白脂肪酶，gpat4. 甘油-3-磷酸盐酰基转移酶4，dgat1. 二酰甘油O-酰基转移酶1，acadm. 中长链酰基辅酶A脱氢酶，β-actin. β-肌动蛋白。
Notes: il-8. interleukin-8, il-10. interleukin-10, tnf-α. tumor necrosis factor α, tgf-β. transforming growth factor β, nf-κb. nuclear factor kappa B, cat.
catalase, sod. superoxide dismutase, pepck. phosphoenolpyruvate carboxykinase, G6pase. glucose-6-phosphatase, pfk. phosphofructokinase, pk. pyruvate
kinase, gk. glucokinase, gs. glycogen synthase, glut2. glucose transporter-2, fbpase. fructose-1,6-bisphosphatase, acc1. acetyl-coa carboxylase, fasn. fatty
acid synthase, atgl. adipose triglyceride lipase, hsl. hormone-sensitive lipase, mgl. monoacylglycerol lipase, pparα. peroxisome proliferator-activated
receptor α, srebp1. sterol regulatory element binding protein-1, lxr-1. liver x receptor, lpl. lipoprotein lipase, gpat4. glycerol-3-phosphate acyltransferase
4, dgat1. diacylglycerol o-acyltransferase 1, acadm. acyl-coa dehydrogenase medium chain, β-actin. β-actin.

分析。H.E 染色结果显示 (图版-1~2)，CON 肝 结合油红 O 染色与 PAS 染色发现 (图版-3~6)，
血窦出现频率高，而 0.10% 牛磺酸组肝血窦减 与 CON 相比，添加牛磺酸后肝脏糖原明显减少，
少，细胞结构完整，细胞界限明显且排列整齐。 且橘红色脂肪滴面积和数量减少。

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
5