Page 37 - 《中国药科大学学报》2026年第2期

学报
Journal of China Pharmaceutical University 2026, 57(2): 163 − 171 163

胰脂肪酶抑制剂筛选方法的研究进展

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张欣艺 ，武晓玉 1,2,3,4* ，陶子豪 ，魏舒畅 1,2,4 ，赵 磊 1,2,3,4 ，段文达 1,2,3,4 ，潘燕龙 ，
迪丽尼尕尔·阿布都艾尼 ，马银云 4
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（ 甘肃中医药大学陇药产业创新研究院, 兰州 730000； 西北中藏药省部共建协同创新中心, 兰州 730000；
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3 甘肃省高校中（藏）药化学与质量研究省级重点实验室, 兰州 730000； 甘肃中医药大学药学院, 兰州 730000；
5 甘肃中医药大学中西医结合学院, 兰州 730000）
摘 要 肥胖及其相关代谢性疾病已成为全球重大公共卫生威胁，其发病率持续攀升，并显著加剧心脑血管疾病、糖尿病等
并发症的风险。胰脂肪酶是将食源性脂质转化成甘油三酯和脂肪酸的关键酶；其活性的有效抑制已成为治疗肥胖症的重要
策略。本文围绕胰脂肪酶抑制剂的筛选方法展开论述，重点归纳并评述了传统筛选法、现代新型筛选法及虚拟筛选法的基本
原理、优缺点及应用现状。针对胰脂肪酶抑制剂筛选方法面临的问题，未来研究亟须走向多技术融合、筛选智能化与中药复
杂体系化的协同创新之路，以开拓新的研究范式。
关键词 胰脂肪酶；抑制剂；对硝基苯酚法；荧光法；固定化酶法；亲和超滤质谱法；虚拟筛选
中图分类号 R965.1 文献标志码 A 文章编号 1000−5048(2026)02−0163−09
doi：10.11665/j.issn.1000−5048.2025092401

引用本文 张欣艺，武晓玉，陶子豪，等. 胰脂肪酶抑制剂筛选方法的研究进展 [J]. 中国药科大学学报，2026，57（2）：163-171.

Cite this article as: ZHANG Xinyi, WU Xiaoyu, TAO Zihao, et al. Research advances in screening methods for pancreatic lipase inhibitors[J].
J China Pharm Univ, 2026, 57(2): 163-171.

Research advances in screening methods for pancreatic lipase inhibitors
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ZHANG Xinyi , WU Xiaoyu 1,2,3,4* , TAO Zihao , WEI Shuchang 1,2,4 , ZHAO Lei 1,2,3,4 , DUAN Wenda 1,2,3,4 ,
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PAN Yanlong , Dilinigaer Abuduaini , MA Yinyun 4
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Gansu Pharmaceutical Industry Innovation Research Institute, Gansu University of Chinese Medicine, Lanzhou 730000; Northwest
Collaborative Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province & Ministry of Education of
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PRC, Lanzhou 730000; Key Laboratory of Chemistry and Quality for Traditional Chinese Medicine of Colleges of Gansu Province,
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Lanzhou 730000; College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000; School of Traditional Chinese
and Western Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China
Abstract Obesity and its related metabolic diseases have become a major global public health threat, and its
rising incidence significantly increases the risk of cardiovascular and cerebrovascular diseases, diabetes and other
complications. Pancreatic lipase is a key enzyme that converts food-borne lipids into triglycerides and fatty acids,
and the effective inhibition of its activity has become an important strategy for the treatment of obesity. This
paper discusses the screening methods of pancreatic lipase inhibitors, and summarizes and reviews the basic
principles, advantages and disadvantages and application status of traditional screening methods, modern new
screening methods and virtual screening methods. In view of the problems faced by the screening methods of
pancreatic lipase inhibitors, future research urgently needs to move towards a collaborative innovation path of
multi-technology integration, intelligent screening and complex systematization of traditional Chinese medicine,
so as to open up new research paradigms.

收稿日期 2025-09-24 * 通信作者 E-mail：wxypzw@163.com
基金项目 国家自然科学基金项目（No.82160457）；甘肃省科技计划项目（科技专员专项）（No.25CXGA022）；甘肃省教育厅：高校
教师创新基金项目（No.2023A-077）；中国科学院兰州化学物理研究所开放课题（HWS202306）