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154 学报 Journal of China Pharmaceutical University 2026, 57(2): 144 − 154 第 57 卷
[38] Serizawa N. Biochemical and molecular approaches for produc- [47] Song C, Zheng XM, Zhou ZR, et al. Enhanced regioselectivity
tion of pravastatin, a potent cholesterol-lowering drug[J]. and catalytic activity on C7 hydroxylation of lithocholic acid to
Biotechnol Annu Rev, 1996, 2: 373-389. produce ursodeoxycholic acid by monooxygenase CYP107D1
[39] Ashworth MA, Bombino E, de Jong RM, et al. Computation- based on semi-rational design[J]. Biotechnol J, 2025, 20(7):
aided engineering of cytochrome P450 for the production of e70070.
pravastatin[J]. ACS Catal, 2022, 12(24): 15028-15044. [48] Yong YF, Liu S, Sakai K, et al. Biosynthesis of reveromycin
[40] Xiong MY, Du ZQ, Fan ZH, et al. Systematic metabolic engi- derivatives by altering the regioselectivity of cytochrome
neering of an industrial Penicillium citrinum for one-step
P450revI[J]. Chem Sci, 2025, 16(28): 13106-13114.
pravastatin production[J]. Metab Eng, 2026, 94: 223-230.
[49] Pan YJ, Li GB, Liu RX, et al. Unnatural activities and mecha-
[41] Thomson RES, D’Cunha SA, Hayes MA, et al. Use of engi-
nistic insights of cytochrome P450 PikC gained from site-spe-
neered cytochromes P450 for accelerating drug discovery and
cific mutagenesis by non-canonical amino acids[J]. Nat Com-
development[J]. Adv Pharmacol, 2022, 95: 195-252.
mun, 2023, 14(1): 1669.
[42] Meng SQ, Ji Y, Zhu LL, et al. The molecular basis and enzyme
[50] Sharma VK, Hutchison JM, Allgeier AM. Redox biocatalysis:
engineering strategies for improvement of coupling efficiency in
quantitative comparisons of nicotinamide cofactor regeneration
cytochrome P450s[J]. Biotechnol Adv, 2022, 61: 108051.
methods[J]. ChemSusChem, 2022, 15(22): e202200888.
[43] Li YM, Yu HW, Ye LD. Protein engineering for enhancing
[51] Yin ZY, Ai JY, Gao JK, et al. From tradition to innovation: the
electron transfer in P450-mediated catalysis[J]. Biotechnol Bio-
transition of P450 enzyme catalysis via light-driven electron
eng, 2025, 122(8): 1966-1981.
transfer[J]. ACS Catal, 2025, 15(15): 13412-13427.
[44] Hajdaś G, Koenig H, Pospieszny T. Recent advances in steroid
[52] Richards L, Jarrold A, Bowser T, et al. Cytochrome P450-medi-
discovery: structural diversity and bioactivity of marine and ter-
ated N-demethylation of noscapine by whole-cell biotransfor-
restrial steroids[J]. Int J Mol Sci, 2025, 26(7): 3203.
mation: process limitations and strategies for optimisation[J]. J
[45] Zhang Y, Cao YX, de Visser SP. Vitamin D(3) activation by cy-
Ind Microbiol Biotechnol, 2020, 47(6): 449-464.
tochrome P450 enzymes: differences between bacterial and hu-
man calcitriol biosynthesis[J]. J Am Chem Soc, 2025, 147(40): [53] Yu JM, Ge JW, Yu HW, et al. Improved bioproduction of the
36898-36910. nylon 12 monomer by combining the directed evolution of P450
[46] Grobe S, Badenhorst CPS, Bayer T, et al. Engineering regiose- and enhancing heme synthesis[J]. Molecules, 2023, 28(4): 1758.
lectivity of a P450 monooxygenase enables the synthesis of ur- [54] Lv FF, Zhang JX, You SP, et al. From traditional to AI-
sodeoxycholic acid via 7β-hydroxylation of lithocholic acid[J]. driven:the evolution of intelligent enzyme engineering for bio-
Angew Chem Int Ed, 2021, 60(2): 753-757. catalysis[J]. Biotechnol Adv, 2026, 87: 108788.

