Page 27 - 《中国药科大学学报》2026年第2期

第 57 卷第 2 期 尹莹莹，等：链霉菌细胞色素 P450 酶的功能及改造和应用 153

[6] Rittle J, Green MT. Cytochrome P450 compound I: capture, sis of himastatin[J]. Science, 2022, 375(6583): 894-899.
characterization, and C-H bond activation kinetics[J]. Science, [23] Zhang HD, Chen J, Wang H, et al. Structural analysis of HmtT
2010, 330(6006): 933-937. and HmtN involved in the tailoring steps of himastatin biosyn-
[7] Sun CX, Wang YC, Hu BD, et al. Establishing an efficient elec- thesis[J]. FEBS Lett, 2013, 587(11): 1675-1680.
tron transfer system for P450 enzyme OleP to improve the [24] Parisi G, Montemiglio LC, Giuffrè A, et al. Substrate-induced
biosynthesis of murideoxycholic acid by redox partner engineer- conformational change in cytochrome P450 OleP[J]. FASEB J,
ing[J]. Angew Chem Int Ed, 2025, 64(19): e202423209. 2019, 33(2): 1787-1800.
[8] Qin XQ, Jiang YP, Chen J, et al. Co-crystal structure-guided [25] Li S, Li Z, Zhang GQ, et al. Functional analysis of the whole
optimization of dual-functional small molecules for improving CYPome and Fdxome of Streptomyces venezuelae ATCC
the peroxygenase activity of cytochrome P450BM3[J]. Int J Mol 15439[J]. Eng Microbiol, 2024, 4(4): 100166.
Sci, 2022, 23(14): 7901. [26] Amaya JA, Lamb DC, Kelly SL, et al. Structural analysis of
[9] Jiang FJ, Wang ZH, Cong ZQ. Tuning the peroxidase activity of P450 AmphL from Streptomyces nodosus provides insights into
artificial P450 peroxygenase by engineering redox-sensitive substrate selectivity of polyene macrolide antibiotic biosynthet-
residues[J]. Faraday Discuss, 2024, 252: 52-68. ic P450s[J]. J Biol Chem, 2022, 298(4): 101746.
[10] Zhao PX, Kong FH, Jiang YP, et al. Enabling peroxygenase ac- [27] Li J, Zhang CD, Wu SW, et al. Cytochrome P450-catalyzed al-
tivity in cytochrome P450 monooxygenases by engineering hy- lylic oxidation of pentalenene to 1-deoxypentalenic acid in pen-
drogen peroxide tunnels[J]. J Am Chem Soc, 2023, 145(9): talenolactone biosynthesis[J]. Eng Microbiol, 2025, 5(2):
5506-5511. 100206.
[11] Fan SX, Cong ZQ. Emerging strategies for modifying cy- [28] Duan L, Jogl G, Cane DE. The cytochrome P450-catalyzed ox-
tochrome P450 monooxygenases into peroxizymes[J]. Acc idative rearrangement in the final step of pentalenolactone
Chem Res, 2024. DOI:10.1021/acs.accounts.3c00746. biosynthesis: substrate structure determines mechanism[J]. J Am
[12] Nebert DW, Adesnik M, Coon MJ, et al. The P450 gene super- Chem Soc, 2016, 138(38): 12678-12689.
family: recommended nomenclature[J]. DNA, 1987, 6(1): 1-11. [29] Wang JB, Xu Y, Chen DD, et al. A bacterial cytochrome P450
[13] Hannemann F, Bichet A, Ewen KM, et al. Cytochrome P450 enzyme catalyzes multistep oxidation reactions in pyrroin-
systems: biological variations of electron transport chains[J]. domycin biosynthesis[J]. Chin J Chem, 2023, 41(19): 2439-
Biochim Biophys Acta, 2007, 1770(3): 330-344. 2445.
[14] Daiber A, Shoun H, Ullrich V. Nitric oxide reductase (P450nor) [30] Song YH, Amaya JA, Murarka VC, et al. Biosynthesis of a new
from Fusarium oxysporum[J]. J Inorg Biochem, 2005, 99(1): skyllamycin in Streptomyces nodosus: a cytochrome P450
185-193. forms an epoxide in the cinnamoyl chain[J]. Org Biomol Chem,
[15] Aldemir H, Shu SJ, Schaefers F, et al. Carrier protein-free enzy- 2024, 22(14): 2835-2843.
matic biaryl coupling in arylomycin A2 assembly and structure [31] Stout CN, Renata H. Total synthesis facilitates in vitro reconsti-
of the cytochrome P450 Ar y C[J]. Chemistry, 2022, 28(2): tution of the C–S bond-forming P450 in griseoviridin biosynthe-
e202103389. sis[J]. J Am Chem Soc, 2024, 146(31): 21815-21823.
[16] He J, Müller M, Hertweck C. Formation of the aureothin [32] Xiao F, Zhou TP, Dong S, et al. Molecular basis for the P450-
tetrahydrofuran ring by a bifunctional cytochrome p450 catalyzed sp3 C–N glycosidic bond formation in staurosporine
monooxygenase[J]. J Am Chem Soc, 2004, 126(51): 16742- biosynthesis[J]. ACS Catal, 2024, 14(19): 14274-14284.
16743. [33] Wang Y, Chen H, Makino M, et al. Theoretical and experimen-
[17] Sun TJ, Liu MY, Li S, et al. Cytochrome P450-catalyzed tal studies of the conversion of chromopyrrolic acid to an antitu-
tetrahydrofuran formation via dual pathways in avermectin mor derivative by cytochrome P450 StaP: the catalytic role of
biosynthesis[J]. ACS Catal, 2025, 15(4): 3295-3305. water molecules[J]. J Am Chem Soc, 2009, 131(19): 6748-6762.
[18] Olano C, Moss SJ, Braña AF, et al. Biosynthesis of the angio- [34] Yu HL, Hu L, Ruan X, et al. Rational mining and engineering
genesis inhibitor borrelidin by Streptomyces parvulus Tü4055: of unique P450 enzymes enable production of diverse dimeric
insights into nitrile formation[J]. Mol Microbiol, 2004, 52(6): tryptophan-containing diketopiperazine alkaloids[J]. ACS Catal,
1745-1756. 2025, 15(21): 18450-18462.
[19] Zhang B, Jin WZ, Zhang YY, et al. A type I/type III PKS hy- [35] Reisberg SH, Gao Y, Walker AS, et al. Total synthesis reveals
brid generates cinnamomycin A-D[J]. Org Lett, 2023, 25(15): atypical atropisomerism in a small-molecule natural product,
2560-2564. tryptorubin A[J]. Science, 2020, 367(6476): 458-463.
[20] Shi YY, Jiang ZB, Hu XW, et al. The cytochrome P450 catalyz- [36] Louka S, Barry SM, Heyes DJ, et al. Catalytic mechanism of
ing C-S bond formation in S-heterocyclization of chuangxin- aromatic nitration by cytochrome P450 TxtE: involvement of a
mycin biosynthesis[J]. Angew Chem Int Ed, 2021, 60(28): ferric-peroxynitrite intermediate[J]. J Am Chem Soc, 2020,
15399-15404. 142(37): 15764-15779.
[21] Yunt Z, Reinhardt K, Li AY, et al. Cleavage of four carbon-car- [37] Li HX, Zhang QB, Li SM, et al. Identification and characteriza-
bon bonds during biosynthesis of the griseorhodin a spiroketal tion of xiamycin A and oxiamycin gene cluster reveals an ox-
pharmacophore[J]. J Am Chem Soc, 2009, 131(6): 2297-2305. idative cyclization strategy tailoring indolosesquiterpene
[22] D’Angelo KA, Schissel CK, Pentelute BL, et al. Total synthe- biosynthesis[J]. J Am Chem Soc, 2012, 134(21): 8996-9005.