Page 140 - 《中国药科大学学报》2026年第2期

学报
266 Journal of China Pharmaceutical University 2026, 57(2): 266 − 274

人脐带源间充质干细胞联合肠道益生菌

促进糖尿病小鼠创面愈合

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靳倩倩 ，张辰阳 ，叶 民 ，沈华超 ，金 亮 1**
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（ 中国药科大学生命科学与技术学院, 南京 211198； 南京医科大学附属明基医院, 南京 210026）
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摘 要 探讨人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hUCMSCs）联合肠道益生菌对糖尿病小鼠
皮肤创面愈合的治疗作用及其潜在机制。采用糖尿病创面小鼠模型，将 25 只 C57BL/6J 雄性小鼠随机分为 5 组，即空白对照
组、模型组、hUCMSCs 治疗组、益生菌治疗组和 hUCMSCs 联合益生菌治疗组。分别于造模后第 0、3、6、9、12 天拍照记录创
面愈合情况，并分析各组创面愈合率的差异；HE 和 Masson 染色观察组织病理学变化及胶原沉积情况；CD31 免疫荧光检测
血管新生情况；Collagen I 免疫组化评估Ⅰ型胶原的形成情况；ELISA 检测创面皮肤组织和血清中抗炎因子（Arg1）及促炎
因子（IL-6、IL-1β、TNF-α）的表达水平。结果显示：造模后第 12 天，与其他组比较，联合治疗组创面收缩最为明显，愈合速度最快；
HE 和 Masson 染色显示，联合治疗组上皮化进程最快且胶原沉积最多；免疫荧光和免疫组化显示，联合治疗组 CD31 和
Collagen I 表达量最多；ELISA 结果显示，联合治疗组创面皮肤组织和血清中 Arg1 表达量高于其他组，同时其 IL-6、IL-1β、
TNF-α 表达量明显低于其他组。以上结果表明：hUCMSCs 与肠道益生菌联合治疗可通过促进血管新生、增强胶原沉积、调控炎
症微环境等机制协同加速糖尿病小鼠创面愈合，其疗效显著优于单一治疗，该研究为糖尿病足的临床治疗提供了新的潜在策略。
关键词 人脐带间充质干细胞；肠道益生菌；糖尿病；创面愈合
中图分类号 R965 文献标志码 A 文章编号 1000−5048(2026)02−0266−09
doi：10.11665/j.issn.1000−5048.2025082803

引用本文 靳倩倩，张辰阳，叶民，等. 人脐带源间充质干细胞联合肠道益生菌促进糖尿病小鼠创面愈合 [J]. 中国药科大学学报，2026，
57（2）：266-274.

Cite this article as: JIN Qianqian, ZHANG Chenyang, YE Min, et al. Human umbilical cord-derived mesenchymal stem cells combined with
intestinal probiotics promote wound healing in diabetic mice[J]. J China Pharm Univ, 2026, 57(2): 266-274.

Human umbilical cord-derived mesenchymal stem cells combined with
intestinal probiotics promote wound healing in diabetic mice
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JIN Qianqian , ZHANG Chenyang , YE Min , SHEN Huachao , JIN Liang 1**
1 School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198;
2 Nanjing Mingji Hospital, Nanjing Medical University, Nanjing 210026, China
Abstract The therapeutic effect of human umbilical cord mesenchymal stem cells (hUCMSCs) combined with
intestinal probiotics on the wound healing of diabetic mice and its potential mechanism were explored. A diabetic
wound mouse model was established, and 25 male C57BL/6J mice were randomly divided into five groups: blank
control group, model group, hUCMSCs treatment group, probiotics treatment group, and hUCMSCs combined
with probiotics treatment group. The wound healing conditions were photographed and recorded on days 0, 3, 6,
9, and 12 after modeling, and the differences in wound healing rates among the groups were analyzed. HE and
Masson staining were used to observe the histopathological changes and collagen deposition. CD31
immunofluorescence was used to detect angiogenesis. Collagen I immunohistochemistry was used to evaluate the
formation of type I collagen. ELISA was used to detect the expression levels of anti-inflammatory factors (Arg1)

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收稿日期 2025-08-28 通信作者 E-mail：shenhuachao2010@163.com
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E-mail：liangjin1975@cpu.edu.cn
基金项目 国家自然科学基金项目（No.82574474，No.82373925）；南京市卫生科技发展专项资金项目（YKK19144）