Page 128 - 《中国药科大学学报》2026年第2期

254 学报 Journal of China Pharmaceutical University 2026, 57(2): 246 − 255 第 57 卷

A Caspase-1 DAPI Merge B NLRP3 DAPI Merge

Control Control

OGD/R OGD/R

Aspirin (100 μmol/L) Aspirin (100 μmol/L)

Indobufen (200 μmol/L) Indobufen (200 μmol/L)
C NF-κB DAPI Merge D GSDMD TUNEL DAPI Merge

Control Control

OGD/R OGD/R

Aspirin (100 μmol/L) Aspirin (100 μmol/L)

Indobufen (200 μmol/L) Indobufen (200 μmol/L)
E F G
Indobufen
Control OGD/R Aspirin (100 μmol/L) (200 μmol/L) 1.0 ## Caspase-1 1.5 Cytoplasma
NF-κB p65
Cleaved- 0.8 1.0
20 kD 0.6 ** **
Caspase1 Relative expression level (ratio of β-actin) 0.4 * Relative expression level (ratio of β-actin)
Cytoplasma 0.5 ##
65 kD NF-κB p65 0.2 0
0
Aspirin
Aspirin
42 kD β-actin Control OGD/R (100 μmol/L) Indobufen Control OGD/R (100 μmol/L) Indobufen
(200 μmol/L)
(200 μmol/L)
H I 1.5 Nucleus
Indobufen
Control OGD/R Aspirin (100 μmol/L) (200 μmol/L) 1.0 ## NF-κB p65

Nucleus Relative expression level (ratio of Histone3)
65 kD **
NF-κB p65 0.5 **
15 kD Histone3 0
Control OGD/R Aspirin Indobufen
(100 μmol/L) (200 μmol/L)

Figure 6 In vitro indobufen reduces HUVEC pyroptosis and regulates NF-κB/Caspase-1/GSDMD expressions after OGD/R ( x ± s, n=3)
A-D: Representative images of A-Casapse-1, B-NLRP3, C-NF-κB, and D-TUNEL/GSDMD in HUVEC cells were detected by IF staining (×200,
scale bar=50 μm). E-I: Western blot analysis of the ratio of Caspase-1 and NF-κB p65 expression in HUVEC cells
#
##
*P < 0.05, **P < 0.01 vs model group; P < 0.05, P < 0.01 vs control group