Page 74 - 《中国药科大学学报》2025年第5期

606 学报 Journal of China Pharmaceutical University 2025, 56(5): 601 − 612 第 56 卷
A Kidney tissue B Kidney tissue C Kidney tissue D Kidney tissue

Cit-H3 18 kD NE 36 kD TF 45 kD TFPI 32 kD
H3 15 kD β-actin 42 kD β-actin 42 kD β-actin 42 kD
ns
ns
1.0 ** * 1.5 ns 1.0 ** ** 1.5 ns
0.8 ** ** 0.8 ** **
Fold of control 0.6 Fold of control 1.0 Fold of control 0.6 Fold of control 1.0

0.4
0.4
0.2 0.5 0.2 0.5
0 0 0 0
Comtrol LPS LPS+Ozone Ozone Comtrol LPS LPS+Ozone Ozone Comtrol LPS LPS+Ozone Ozone Comtrol LPS LPS+Ozone Ozone

E Kidney tissue F Kidney tissue G Kidney tissue
MMP9 92 kD PAR2 55 kD p53 53 kD
β-actin 42 kD β-actin 42 kD β-actin 42 kD
ns ns
1.5 1.5 1.5 ns
** * ** *
** **
Fold of control 0.5 Fold of control 0.5 Fold of control 0.5
1.0
1.0
1.0

0 0 0
Comtrol LPS LPS+Ozone Ozone Comtrol LPS LPS+Ozone Ozone Comtrol LPS LPS+Ozone Ozone

H Control LPS LPS+Ozone Ozone

Merge

TF

Cit H3

DAPI

Figure 2 Ozone alleviated sepsis-induced renal microcirculatory embolic injury ( x ± s, n=6)
A-G: Protein levels of Cit-H3, neutrophil elastase (NE), TF, TF pathway inhibitor (TFPI), matrix metalloprotein (MMP) 9, protease-
activated receptor (PAR)-2, and p53 in the kidney were evaluated after 24 h by Western blot; H: Representative images of co-staining for
Cit-H3 (green), TF (red), and DAPI (blue). Scale bar=100 μm
*P<0.05, **P<0.01; ns: not significant