Page 46 - 《水产学报》2026年第3期

3 期 刘怡龙，等：“斑马蛤 2 号”性腺发育及生化营养成分周年变化 50 卷

Gonadal development and annual variations in biochemical nutritional
components of Ruditapes philippinarum “Zebra II”

*
LIU Yilong , LI Cuicui , LÜ Jiapeng , HUO Zhongming , QIN Yanjie , WANG Hua ,
*
GU Jing , YANG Feng , YAN Xiwu
(Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, College of Fisheries and Life Science,
Dalian Ocean University, Dalian 116023 China)

Abstract: To understand reproductive characteristics and nutritional composition changes of the “Zebra Ⅱ”, and to explore its
cultivation effectiveness in the tidal flats of Zhuanghe waters, this study investigated and analyzed the seasonal variations in
gonadal development and nutritional composition, as well as the annual amino acid profile, of the “Zebra Ⅱ” cultivated in the
waters off Zhuanghe. Gonadal tissue sectioning and sex steroid hormone level analyses revealed two annual reproductive peri-
ods for “Zebra II”: May-June and September-October. Gonadal development was categorized into five phases: proliferative
phase (January-March), growth phase (Late March-Early May), maturation phase (May-June), release phase (June-July/Septem-
ber-October), and quiescent phase (late July-early August/November-February). Sex steroid hormone levels peaked during the
June maturation phase: estradiol (125.62 ng/L), testosterone (35.19 nmol/L), with a sex ratio of 1.04∶1 (P > 0.05). Annual
fluctuations in routine nutritional components of “Zebra II” soft tissue were as follows: moisture 72.23%-84.34% (annual aver-
age 80.30%), crude ash 9.52%-14.75% (annual average 11.98%), crude fat 2.70%-11.53% (annual average 8.40%), crude pro-
tein 51.60%-64.66% (annual average 57.85%), and muscle glycogen 7.34-11.50 mg/g (annual average 9.20 mg/g). Seventeen
amino acid components were detected in “Zebra II”, with a total amino acid content (TAA) of 630.45 mg/g. Among these,
glutamic acid (Glu), aspartic acid (Asp), and glycine (Gly) exhibited high abundance levels. Evaluated using amino acid scor-
ing (AAS) and chemical scoring (CS), methionine (Met) was identified as the primary limiting amino acid. Research indicates
that “Zebra II” can achieve stable reproduction through “double spawning per year” in the Zhuanghe coastal waters, with good
year-round gonadal development. It is a high-protein, nutritionally valuable aquaculture bivalve well-suited to the low-temper-
ature environment of northern tidal flats. These findings provide a reference for promoting “Zebra II” aquaculture and utilizing
northern tidal flats.
Key words: Ruditapes philippinarum; "Zebra II"; gonadal development; biochemical nutrients; seasonal variations
Corresponding authors: HUO Zhongming. E-mail: huozm@dlou.edu.cn;
GU Jing. E-mail: gujing@dlou.edu.cn
Funding projects: Liaoning Provincial Science and Technology Plan Project (2022-BS-271); Liaoning Provincial Education
Department Project (LJ212410158053); Liaoning Basic Research Funds for Undergraduate Universities (2024JBQNZ019);
Liaoning Provincial Major Science and Technology Special Project (Germplasm Innovation Program)(2024JH1/11700010);
Central Government Subsidy Project for Liaoning Fisheries(2024, 2025); Liaoning Provincial Department of Science and Tech-
nology, Joint Program of Liaoning Provincial Science and Technology Program (Program for Technological Breakthrough)
(2024JH2/102600078); Modern Agroindustry Technology Research System (CARS-49); Outstanding Young Scientific and
Technological Talents Foundation of Dalian (2021RJ09)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
13