Page 42 - 《水产学报》2026年第01期

1 期 水 产 学 报 50 卷

CFU/尾的鰤鱼诺卡氏菌 NSFS01 株腹腔注射感染 头肾和脾脏中 IL-12 较健康对照组的表达变化。
大口黑鲈，通过转录组测序分析不同感染时间点 在大口黑鲈头肾中，感染后24 h，p40a、p40b、

α1 α2
智人`Homo sapiens p35 . TTT TTT TT TT TT
1 1 0 2 0 3 0 40 5 0 6 0 7 0 8 0 9 0 10 0 11 0
智人`Homo sapiens p35 .......MCPARSLLL T V DH S R..NL ATPDPGMFP LHHSQ RA N QKAR.QTLEFYPCTS EI H .DITKDK EA L LELTKNESCLNSRETSFITNG LA
VA L LL L LA PV C N L VS ML E D E TSTV P S
L C C S
小鼠`Mus musculus p35 L C C P
LA L LL L LA PV C RN L MV E D E TSTL P S
.......MCQSRYLLF T A NH S R..VI SGPAR.... LSQS KTTDD KTAR.EKLKHYSCTA DI H .DITRDQ KT L LELHKNESCLATRETSSTTRG LP
V LL L LL L R L AN AL E D E SNTI N
L C C L
原鸡`Gallus gallus p35 MAEHGIGIGSRAARLG GRCV AA C LPSTWA PPPAHNLAKGLNCS A AA E LKVQKQRTLGFECTL EV L .DVTNSQ KS TSQDPGP...............G PV
热带爪蟾`Xenopus tropicalis p35 .....MASRRAGSWVI V FCCQSKF QSKPVL DLSNSVDLVK FKSS NV ET LEIK.SNYKDFNCSY EI MRNVIAKDKM I TS I SSLSKD..............HM QS
L C C L
LA L A G C KS V VN L D D N L P
斑马鱼`Danio rerio p35a L C C H
M I IV M LA PV C RS L LT IM D E T T P S
................. K C IS A LALCS PPPDSQECV SPAA SA H EKEGKMKEQDLFAGFNCT L .AQMTPH H SSV Q SVSAN...............S SA
草鱼`Ctenopharyngodon idella p35a ................. K C FC V LAMGS P.QNTRQGS TSAA SN H EKEGKMKHQDLFAGFNCT L .ARMIPY H SV Q IATDN...............T SN
M I VL V VA PV C RS L LS IM D E T TA P S
L C C S
大口黑鲈`M. salmoides p35a L C C I
......MPFIKLYFTP L L SCPLWQVSQSL MTKGPMMDS VLYA EN D TQN......NLFSGI CKKQS.VELNME PSV A KE..................S SG
AL L VL PV C RT L IT AL D TST P T
1 2 3 1
智人`Homo sapiens p35 α3 α4 α5

120 130 14 0 15 0 160 17 0 18 0 190 200 21 0
智人`Homo sapiens p35 TSF L S K Y L ML VI ELMQ L V Y KI L LLH F I V I VM L
RK MMAL S YE M QVEFKTMNAKLLMDPKRQIF DQN A D A NFNSET PQKSSLEEP...DF KT K I A R A D S NAS...
C I DL C R T R Y .
小鼠`Mus musculus p35 QK LMMTL G YE M QTEFQAINAALQNHNHQQII DKG V D S NHNGET RQKPPVGE ...DP RV K I A ST V G S A...
C I DL C R T R Y .
TS L S K Y L ML AI ELMQ L L A Y KM L LLH F V IN VM L S
原鸡`Gallus gallus p35 ES LDMSK QG YE T KAE GNL.............KDLR TS D A QPRSPA PQPSPSTT ...G QG R G A C A G G SALTAE
ST L K Y L VL I DMMQ L M L SF RM L VLH F L V I ML L
C I DL C R T R Y M
热带爪蟾`Xenopus tropicalis p35 F L K F L L AI LMQ L SF KL VI Y L I S VL L S
NKAE DEAK KA HA V KDE QES..............TPS DA SQ D KVQDTEESNVKKELCS.... DC KQ R LF Q T F N R T...
C I DL C R T R Y .
斑马鱼`Danio rerio p35a C I DL C R T R Y K
SNF V N R Y L L VI A LIN L A V SF RV L LK F I I VN AL I S
RS SAVE K RG Y DLL NSYKPSTQ.......E SP. T NTQ C NQRCES EGVFPQWR WKGS DD S KT G H S A A GEHR
RS SETE R SG Y DLL NSYQPSVS.......D GP. T TK LC KANGDA EGEFPQWV WSGS DD S KT G H A A A GDHR
C I DL C R T R Y K
草鱼`Ctenopharyngodon idella p35a SSF L N H Y L L VM A DLM L A V SF RL L LK F V I MN AL I S
大口黑鲈`M. salmoides p35a VK E DQEM T RK H YKF AAQPDPDS.......L GPT FS R ENCFTWSLPTNMASKEVA DRPS DE S K G Q T A N GEHT
S F L N H Y L L VL L ELM V TY RL L VLK F V I IN VL M S
C I DL C R T R Y K
3 2
(a)
β1 β2 β3 β4 β5 β6 η1 β7 β8
智人`Homo sapiens p40 TT TT TT .. TT
1 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 0 10 0
智人`Homo sapiens p40 MCHQQLVISWFS FLASPLVA......IWELKKDVY ELDWYPDAPGEM V T .DTPE........EDG TWTLDQSSEVLGS KT T QVKEFGDA H..KGGEV SH L HK
C C K
LV VV V L I G L I GQYT L S LLL
小鼠`Mus musculus p40 MCPQKLTISWFA LLVSPLMA......MWELEKDVY EVDWTPDAPGET N T .DTPE........EDD TWTSDQRHGVIGS KT T TVKEFLDA H..KGGET SH H HK
C C K
IV VV V L I G L I GQYT L S LLL
原鸡`Gallus gallus p40 ..MSHLLFALLS SFAALLEA......QWKLRENVY ESEWNDETPAKK K T .DTS.........DEA PVYWKKGTELKGT KT TTEVKEFPDA LSAKTHEI SY FF TK
LL VI V L L G L GNYT I S LI
C C V
热带爪蟾`Xenopus tropicalis p40a MPLIVPSLALAL GMSCCQAT......ELMEWPLQY LSDSD......DYT E DTKER..........N RWLAPSWTCSAPV KA Y SDIQLPCA L LSARKGLL WSMY AD
LV AV L I G L I GQY L LLI
C C A
LM VV V V G L GNFT I VLL
C C L
热带爪蟾`Xenopus tropicalis p40b ..MEYLKFGLLL VRVNTLYG....LWDLSVLKNTY DISES..GEGEN IFE PVVEE.........TK YWLIKGNQ...RP RI RTHVMEDIDA HR.LTGEL DYKV NT
斑马鱼`Danio rerio p40a ...MCQLTRVIL CLSIVRLS......ALNFFPGKFK GERG......TD I T HTSEHEITWKHQ.DAHSISKISGSEFEKIS RT T MDLDEDQI WS..QQGLEDY Y DK
C C T
LL V V L G L L GNYT T LLL
V L I G I V GNFT L T LLV
C C V
斑马鱼`Danio rerio p40b ........................................MQEL.SSF..T P H GEQYE........GEQ HWEKNGKS.ILET NR N TINGLL.G HR.PNRDF NY I HP
C C T
ML VV V L I G I I YS L T VLA
草鱼`Ctenopharyngodon idella p40a ..MMFFLLFGTL LQSSAVGSIKTAESYWTLKPNVL NVEVGKDVNIAQ P I GEVYE........GQN TWIKNKDEKLEAQ NR M TVEGWK.GAD FN.KEGSY NH L QW
草鱼`Ctenopharyngodon idella p40b AV VL V L I G I V GNFT L T LLV
..MNKIVFFILQ LQLAGSTS......LRFLKPNVE EVSDL.SSS..E S H GEQYE........GEQ HWERNGES.ISET NR T TIDGLR.G HR.ANGDL SY L HP
C C V
草鱼`Ctenopharyngodon idella p40c LV I V L G L V GNYT T LL
...MGLLMRFIV CLSITRMS......ALKFFPEKFE GEKG......TS T T KTNKDSVTWKRE.DALGIVDIVESDYEKLS RH T IDLQKDQI WS..DTGLEDY YR DK
C C S
大口黑鲈`M. salmoides p40a AA VL V L V G V V GNYT L T IMI
..MHALFLMVLC LSCASSDSN..QDNIQTLMDNVL RVPYG.QGTRVY P T GEAYQ........DQS FWKKNGESQPALQ NQ N LVEEMD.G HLGPDGEY NH V QL
C C D
大口黑鲈`M. salmoides p40b ..MKLFVFGIVC FLQVSCQN....PTSHWTLLPHTL EVDG....TLGQQP S LESPEELTRRDIKSQD FWRKNGVQEAQRGNSY VQLEESLG.G HS.EDGSL NH V QE
C C E
A VV L I L GNYT L T ILI
大口黑鲈`M. salmoides p40c LL VV V L V L V G YS L T LLL
C C N
..MKPVSLWILG CTSLTGAH......ELKDFPENF AKRSD.....AN T A GTKTNEPVTWKF.DGV MEDVFFEDNVQQHNQS T SELQPPML K WR..GGEM SS Y EA
1 1
免疫球蛋白 C2 型结构域 (23~106 aa)
immunoglobulin C-2 type domain
β9 β10 β11 β12 β13 β14 β15 β16
智人`Homo sapiens p40 TT TTT .. TT ....... . TT TT T.T
11 0 12 0 13 0 14 0 15 0 16 0 17 0 18 0 19 0 20 0 21 0
智人`Homo sapiens p40 E.DGIWSTDILKDQKEPKNKTF R E K GR.. T W LTT.ISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNK.......EYE.YS ECQEDSA PAA SLP E M D VHK.
L A NYS F W V C E I V V A
C C E L
L A NYS F SW V C E I L L A
C C E N
小鼠`Mus musculus p40 E.NGIWSTEILKN...FKNKTF K E P GR.. T LVQ.RNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQR.......DYEKYS SCQEDVT PTA TLP E A E RQQ.
原鸡`Gallus gallus p40 DSNGQMIRSILKSYK.EPSKTF K E K GI.. T MTENESPSVKFTIRSLKGSQGD..VTCSSP......VARTDK.......SVTEYT QCQKENY P A HQPTE F E IDE.
L A NYS F SW A C F E M L V
C C E V
热带爪蟾`Xenopus tropicalis p40a V V S T S C F I V V V
C C E K
S..................LLD T F E H SS.TLH ADID.EEEGILRARASVSSTDMPQWYTATIDGTLS.....................FTLSMDKF A E QMEP L E Q ATE.
S.........HLNQRLLPGKQP Q E Q GH.. K KRIKKHVQYIFEAQRGNYT.....ITCEKPSENN.................RKYE NCHDNES Q G SHN N L H LDE.
I A NYS F TW V C Y E I I L I
热带爪蟾`Xenopus tropicalis p40b C C E K
C C E C
L A SFS SW V Y E L I A
斑马鱼`Danio rerio p40a K...............ETTAFN K T E CTERIK RSD.AFTAFRLRNSRDNGK......WVSESVDG...................... FYLPHSTDS S SER Q TGE LSA.
斑马鱼`Danio rerio p40b C C E F
V A NYN F SW L C Y E I L L V
K...FHKALLLKQSS..SKE.F S S R GQ.. H KWH.HERT.HKAVVYFAAIRNSSDLNCTLDSNIS....................E TCIDKDY P S PLS N T L RNM.
草鱼`Ctenopharyngodon idella p40a I NY F W I C Y E I L I
......FKKIIKNIP..EKG.Y H STN GGS.. Q F TWS.LNRDGHVAHIKATRPHSGSNISCSLDSKGQ....................S TCLDHDY P S VYR N T YFRSS.
C C E F
C C E Y
K...FSKGGVLIKSS..DGE.F S T R GQ.. H KWH.QERT.KRAVVYFTALRNSSLINCTLDSDIS....................G TCIDKEY P S PRS N T F RNL.
草鱼`Ctenopharyngodon idella p40b V A NYN F SW L C Y E I L L V
草鱼`Ctenopharyngodon idella p40c I A TFS TW V Y E L I A
K...............EATGSN N T E CSERIK TSD.EFTAFRLRNTRDDGD......WVSQSVDG...................... FFLPHSTNS S SER L TGE VST.
C C E C
大口黑鲈`M. salmoides p40a P...DNRTVILEKKS..PEEGH H S L KGS.. H TKT.KSRS.NAAVLLVKAERYLEKIPCELNADGS....................G HCQDAN. P K QHR S T Y HID.
C C E S
I A NY F TW V C Y E I L I I
L A NYN F SW I C Y E I I V V
C C E F
大口黑鲈`M. salmoides p40b E...TKRRKILVKSD..QED.Y K S Q GE.. H TWH.SRRVGKVAFIKARRVTDSNDIQCSVDTSGQRWTCSSGQRHFGCSVDDSGHR LCLDKQH P T SQQ H S Y KTEH
大口黑鲈`M. salmoides p40c C C E F
D...............EELDSFFS R K HCN.. S K TQS.EYTAVRIGLGQDCSEGRKSCHWVSSSEQLP.................GGGFQFELPHSLSP A SSM E T E ITN.
A SY F W Y E L L A A
2 2 3 3
IL-12p40 C 端结构域 (126~216 aa)
IL-12p40_C domain
β17 α1 β18 β19 β20 β21 β22 β23 β24
智人`Homo sapiens p40 TT ... TT TT TT
22 0 23 0 24 0 25 0 26 0 27 0 28 0 29 0 30 0 31 0 3 2 0
智人`Homo sapiens p40 KYENY SS DPPKN QLKPLKNSRQ...VEVSWEY D S H S T C QVQGKSKREK........KDRVFTDKT....SATVICRKNASIS AQ RYYS S E ASVP
I P P W S F D C
T FFIRD IK L T TP Y L F V VR S WS W
小鼠`Mus musculus p40 KYENY TS DPPKN QMKPLKNS.Q...VEVSWEY D S H S K F RIQRKKEKMKETEEGCNQKGAFLVEKT....STEVQC.KGGNVC QAQ RYYN SC K ACVP
S FFIRD IK L S TP Y L F V V S S W
I P P W S F D C
原鸡`Gallus gallus p40 EYENY SS DPPQ.CQYASTNGT.......VTWTY K S K T R KVESTKKYKS........KVYDADEQS....IQIPKTGPKDKIS QAR RYYN S E TL.
I P P W S F D C
T FFIRD IK T TP Y PL F V V S WS WS
热带爪蟾`Xenopus tropicalis p40a RFMSL KL SPPEN NVLKHNRQ.....LLISWNY TP S YA L E NMIYRNDTEEQIKVEGAQVL..............AVSANVRTIKG CR LYYR E P QGI
I P P W S F D E
N YYIRD VK M N Y PM F L R S WS WT
热带爪蟾`Xenopus tropicalis p40b I P P W S F D H
RYENH LT M T DPPAE KISSTSLT.........WSY K C VH I N NITKGNKSPEHHQGIDVPEMP.............RPPNGKFSFC QAR MYYN S E CSK
S F LRD K L T N F PL F V V S WS WS
斑马鱼`Danio rerio p40a CYMKTDYS ANPNISICRVKDEGSDEQITEVEVKP S P Q THQ QYENRDDGMLKTKEWEKDSK.............VTVKGLIRKLR CR PLLV Q E PWT
I P P W S F D N
FFLRD VK S QP F PL I AR S WS WT
斑马鱼`Danio rerio p40b RLEEHHRT ...DK GITKIQD......NEFEWRP Q SF C S E KVVPRSHDCDYTGNRVEQNE..TNKTH....YKVNSK.KPYTFC AQ PLTKAV D HHH
FFIRD VK V T P F PL Y I IR WS WS
I P P W S F D Q
草鱼`Ctenopharyngodon idella p40a VVEAY TK M ...DM NISRINQ......TSVEVGY H N S T Q KEIRCPKRTKCDCSKPNSPETYLTQSH.....QLPVT.KGRAVC AR EFCN S E LYK
I P P W S F D C
N FYI D VR V S TP Y PL F V VR S WS WN
草鱼`Ctenopharyngodon idella p40b RLEEH RT ...DK SITMIED......DVFEWRP Q SF C S E KVVPNNHDCDFTGNRVEKME..TNETH....YKVNIK.KSYTFC AQ PLTNRV DR HHH
Q FFIRD AK V T P F PL Y V VR WS S
I P P W S F D K
草鱼`Ctenopharyngodon idella p40c CYMKTDYS DDPNISICTVKNKGSDKQIIEVEVMP S P Y KHQ QYQIRHNGELKTKEWEEGSK.............IAVEGQISKLR CR MLLL Q E TWK
I P P W S F D N
FYLRD VK S QP F PL I VR S WS WT
大口黑鲈`M. salmoides p40a RLEAY KT ...AK PNLRISDG.....TVFSWNY E EK C Q Q KVVKAVEDPKHPCNSEGHIMGNITETK....YEVNVKTKKYVFC AQ KHTSGP GH HCI
T FYLRE VR L S P F GL F V VR F WS
I P P W S F D V
大口黑鲈`M. salmoides p40b LVENY KH S ...DK MISTVNT......TMIEWSY R S F T Q AQLKKG.CEKCDNPCTDSKATKILTAHSTDICQFEVKRKTKAVC AK ALCN P E HIR
I P P W S F D L
S FYL E VK V S SP Y PL F I VR S WS WS
大口黑鲈`M. salmoides p40c T FYLRD V V S TP F L I VR S WS WT
I P P W S F D N
YILRR KR Q DSPRI KCQEVEQDLN.....VTIDP S S H S EHE EYVFKDDGMTG.....RSLS.............LLIPKRISKLR SR SLVF A Q PWK
4 4
纤维连接蛋白 III 型结构域 (237~328 aa)
fibronectin type 3 domain
智人`Homo sapiens p40
智人`Homo sapiens p40 S.............................................................
小鼠`Mus musculus p40 RVRS..........................................................
原鸡`Gallus gallus p40 R.............................................................
热带爪蟾`Xenopus tropicalis p40a TPLCK.........................................................
热带爪蟾`Xenopus tropicalis p40b NKPRNKQAKKQKKKKKA.............................................
斑马鱼`Danio rerio p40a VSDRANGNNGRLDMKKGKKMRNRQKKHQRNRQKNHQRNHQKNHQNKKKHQNNQQKHLNWAKM
斑马鱼`Danio rerio p40b ..............................................................
草鱼`Ctenopharyngodon idella p40a RNRKNKKQRNQEPKLMML............................................
草鱼`Ctenopharyngodon idella p40b KKHRHTSEQ.....................................................
草鱼`Ctenopharyngodon idella p40c VN............................................................
大口黑鲈`M. salmoides p40a NKDVVTC.......................................................
大口黑鲈`M. salmoides p40b KNKKKKNNRHRKNKQ...............................................
大口黑鲈`M. salmoides p40c VR.........TGKRNQYKCRNSAKPCCPALPSWSLNSCKKRWKKKRIKDPNRSQTS.....
(b)
图 1 大口黑鲈 IL-12 蛋白序列多重比对
(a) 不同物种 IL-12p35 蛋白多序列比对结果，(b) 不同物种 IL-12p40 蛋白多序列比对结果。红色区域标注为保守位点 (identity≥90%)，黄色
区域标注为同源性≥70% 的区域；序列与上方的序列标识图相对应，标识图中字母高度表示对应位点氨基酸的保守概率 (高度越高，保守
性越强)。黑色虚线框表示不同结构域，框下方标注相应结构域名称；序列上方螺旋与箭头对应蛋白结构中出现的 α 螺旋和 β 折叠。
Fig. 1 Multiple alignment of IL-12 protein sequence of M. salmoides
(a) multiple sequence alignment results of IL-12p35 proteins across different species, (b) multiple sequence alignment results of IL-12p40 proteins across
different species. “red shading” indicates conserved residues (sequence identity≥90%); “yellow shading” highlights regions with sequence identity≥
70%; the alignment corresponds to the “sequence logo” above, where the height of each letter reflects the conservation probability of the amino acid at
that position (higher letters indicate stronger conservation); “black dashed boxes” outline functional domains, with domain names labeled below each
box; the helices and arrows above the sequence correspond to the α-helices and β-sheets present in the protein structure.
https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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