Page 109 - 《水产学报》2025年第11期

夏宁，等 水产学报, 2025, 49(11): 119409
10.0 16.20

30 生物过程
biological process 12.15
分子功能
7.5
molecular function
−lg (P value) 20 差异蛋白占比/% percent of proteins 5.0 8.10 蛋白数目/个 no. of proteins

10

down up 2.5 4.05
0 None None 0 0
−5.0 −2.5 0 2.5 5.0 7.5 1 2 3 4 5 6 7 8 9
log 2 FC (polyIC/PBS) GO 分类
(a) GO term
(b)
1
2
3
4
5 校准值−lg(P value)
3.0
6 7 8 2.5
2.0
KEGG 功能通路 KEGG functional pathways 10 9 数目/个 count
1.5
1.0
11
12
2
13
14
15
16 3 4 5 6
17 7
18
19
20
0.4 0.6 0.8
比率
ratio
(c)
图 1 泛素化差异蛋白鉴定
(a) PBS 对照组与 poly(I:C) 刺激组泛素化差异蛋白火山图。(b) PBS 对照组与 poly(I:C) 刺激组泛素化差异蛋白 GO 富集结果柱状图；1. 类异
戊二烯生物合成，2. 脂质代谢过程，3. 细胞脂质代谢过程，4. 一元羧酸代谢，5. 脂肪酸代谢，6. 磷酸吡哆醛结合途径，7. 肽酶活性，8. 蛋
白质复合物结合，9. 酰基辅酶 a 脱氢酶活性。(c) PBS 对照组与 poly(I:C) 刺激组泛素化差异蛋白 KEGG 通路富集结果气泡图；1. 不饱和脂
肪酸的生物合成，2. 脂肪消化吸收，3. 矿物质吸收，4. 硒化合物代谢，5. 近端小管碳酸氢盐回收，6. 脂肪酸延伸，7. 基底切除修复，8. α -
亚麻酸代谢，9. 胆汁分泌，10. ABC 蛋白转运，11. 胰岛素分泌，12. 甘油磷脂代谢，13. 脂肪酸代谢，14. 醚脂质代谢，15. 醛固酮调节钠重
吸收，16. 2 型糖尿病，17. 醛固酮的合成和分泌，18. PPAR 信号通路，19. 嘌呤代谢，20. 谷胱甘肽代谢。
Fig. 1 Identification of ubiquitinated differential proteins
(a) volcano plot of ubiquitinated differential proteins in PBS control versus poly(I:C)-stimulated groups. (b) histogram of GO enrichment results of ubi-
quitinated differential proteins in PBS control versus poly(I:C)-stimulated groups; 1. isoprenoid biosynthetic process, 2. lipid metabolic process, 3. cellu-
lar lipid metabolic process, 4. monocarboxylic acid metabolic metabolic, 5. fatty acid metabolic process, 6. pyridoxal phosphate binding, 7. peptidase
activity, 8. protein complex binding, 9. acyl-CoA dehydrogenase activity. (c) bubble plots of ubiquitination differential protein KEGG pathway enrich-
ment result in PBS control versus poly(I:C)-stimulated groups; 1. biosynthesis of unsaturated fatty acids, 2. fat digestion and absorption, 3. mineral
absorption, 4. selenocompound metabolism, 5. proximal tubule bicarbonate reclamation, 6. fatty acid elongation, 7. base excision repair, 8.
alpha−Linolenic acid metabolism, 9. bile secretion, 10. ABC transporters, 11. insulin secretion, 12. glycerophospholipid metabolism, 13. fatty acid meta-
bolism, 14. ether lipid metabolism, 15. aldosterone-regulated sodium reabsorption, 16. type 2 diabetes mellitus, 17. aldosterone synthesis and secretion,
18. PPAR signaling pathway, 19. purine metabolism, 20. glutathione metabolism.

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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