Page 111 - 《水产学报》2025年第11期

夏宁，等 水产学报, 2025, 49(11): 119409

65.4
60
生物过程 biological process
9 50 分子功能 molecular function 54.5
43.6
40
−lg( P value) 6 差异蛋白占比/% percent of proteins 30 32.7 蛋白数目/个 number of proteins

21.8
20
3
down up 10 10.9
0 none none
−2.5 0 2.5 5.0 7.5 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 0
log 2 FC (polyIC/PBS) GO 分类
(a) GO term
(b)
1
2
3
4
5 6 7 校准值−lg(P value)
1.7
KEGG 功能通路 KEGG functional pathways 10 8 9 数目/个 count
1.5
1.3
1.1
11
12
2
13
14
15 4 6 8
16
17
18
19
0.3 0.4 0.5 0.6
比率
ratio
(c)
图 2 磷酸化差异蛋白鉴定
(a) PBS 对照组与 poly(I:C) 刺激组磷酸化差异蛋白火山图。(b) PBS 对照组与 poly(I:C) 刺激组磷酸化差异蛋白 GO 富集结果柱状图；1. 翻译起始，
2. 细胞蛋白代谢过程的调控，3. 基因表达，4. 大分子代谢过程，5. 翻译，6. 细胞大分子代谢过程，7. 蛋白质代谢过程，8. 细胞蛋白质代谢过程，
9. 细胞过程，10. 脂质分解代谢过程，11.蛋白质修饰调节过程，12. 磷酸盐化合物代谢过程，13. 细胞代谢过程，14. GTP 结合，15. RNA 结合相
关翻译因子活性，16. 翻译起始因子活性，17. RNA 结合。(c) PBS 对照组与 poly(I:C) 刺激组磷酸差异蛋白 KEGG 通路富集结果气泡图；1. 卵巢
类固醇合成，2. 刺猬信号通路-果蝇，3. 查加斯病 (美洲锥虫病)，4. 乙醚脂质代谢，5. 内分泌及其他因素调节的钙重吸收，6. 黄体酮介导的卵母
细胞成熟，7. 多物种长寿调节途径，8. 含血清素的神经突触，9. 甲状腺激素合成，10. 长期抑郁症，11. 谷氨酸突触，12. 胰高血糖素信号通路，
13. 肾细胞癌，14. 酒精依赖通路，15. 血管平滑肌收缩，16. 血小板活化，17. 催产素信号通路，18. MAPK 信号通路，19. RNA 运输。
Fig. 2 Identification of phosphorylated differential proteins
(a) volcano map of phosphorylated differential proteins in PBS control versus poly(I:C)-stimulated groups. (b) histogram of enrichment results of phos-
phorylated differential protein GO in PBS control versus poly(I:C)-stimulated groups; 1. translational initiation, 2. regulation of cellular protein metabolic
process, 3. gene expression, 4. macromolecule metabolic process, 5. translation, 6. cellular macromolecule metabolic process, 7. protein metabolic process,
8. cellular protein metabolic process, 9. cellular process, 10. lipid catabolic process, 11. regulation of protein modification process, 12. phosphate-containing
compound metabolic process, 13. cellular metabolic process, 14. GTP binding, 15. translation factor activity, RNA binding, 16. translation initiation factor
activity, 17. RNA binding. (c) bubble map of enrichment results of KEGG pathway in PBS control versus poly(I:C)-stimulated groups; 1. ovarian steroido-
genesis, 2. hedgehog signaling pathway-fly, 3. chagas disease (American trypanosomiasis), 4. ether lipid metabolism, 5. endocrine and other factor-regulated
calcium reabsorption, 6. progesterone-mediated oocyte maturation, 7. longevity regulating pathway-multiple species, 8. serotonergic synapse, 9. thyroid hor-
mone synthesis, 10. long-term depression, 11. glutamatergic synapse, 12. glucagon signaling pathway, 13. renal cell carcinoma, 14. alcoholism, 15. vascular
smooth muscle contraction, 16. platelet activation, 17. oxytocin signaling pathway, 18. MAPK signaling pathway, 19. RNA transport.

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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