Page 114 - 《水产学报》2025年第11期

夏宁，等 水产学报, 2025, 49(11): 119409

表 4 脂质代谢相关泛素化修饰差异蛋白表达及修饰位点
Tab. 4 Lipid metabolism-related ubiquitinated differential proteins expression and modification sites
蛋白 蛋白全称 变化 差异倍数 修饰肽段序列 氨基酸位点
protein full name of protein change FC modified sequence AA site
ACOX1 peroxisomal acyl-coenzyme A oxidase 1 down 0.312 52 LVEIAAKNLQAELQHSK K7
HADHA trifunctional enzyme subunit alpha, mitochondrial down 0.312 60 GLMQKIQDYVMSYPFVR K5
APT2 acyl-protein thioesterase 2 down 0.334 48 AAENVKAIIDHEVK K6
FPPS farnesyl pyrophosphate synthase down 0.336 25 LQKLIQLHAK K3
HMGCS hydroxymethylglutaryl-CoA synthase down 0.348 31 ASSELFENKTK K9
LPCAT1 lysophosphatidylcholine acyltransferase down 0.357 54 TALGVGKLK K7
FAS fatty acid synthase down 0.450 61 TSEKLGDQLR K4
FLCQQKPVMSSFVLAEK K6
DAGLα sn1-specific diacylglycerol lipase alpha up 3.074 74 GMVYSAEYIKK K10
ABCA1 phospholipid-transporting ATPase ABCA1-like up 2.979 47 VAGPDPDLQPVMKFIEYELPGSTLK K13
PLD1 phospholipase D1-like up 2.709 07 AILELDGYLSKPGLEKEDPAR K16
VLCAR very-long-chain 3-oxoacyl-CoA reductase-B-like up 2.583 24 LDEVSKDIESK K6
ACAA1 3-ketoacyl-CoA thiolase, peroxisomal up 2.480 52 STVEKMGLPVFGVLR K5
cPLA2 cytosolic phospholipase A2-like up 2.357 15 YGTFMTPDLFGSKFFR K13
EKQDSFALGSQQK K2
m-AST aspartate aminotransferase, mitochondrial up 2.335 13 VGGFTVVCADAEEAKR K15
DGKz diacylglycerol kinase zeta-like up 2.269 52 VVCDGTDLTSKVQDLK K11

的磷酸化。实际上，糖基化修饰在脂质代谢调 20(3): 137-155.
控中同样具有重要作用，例如 O-糖基化可增强 [ 2 ] Senkal C E, Salama M F, Snider A J, et al. Ceramide is meta-
HeLa 细胞中新生脂肪酸合成酶 (FAS) 的活性 ， bolized to acylceramide and stored in lipid droplets[J]. Cell
[32]
但是本研究在 poly(I:C) 刺激后的巨噬细胞中未 Metabolism, 2017, 25(3): 686-697.
筛选到脂质代谢相关蛋白的糖基化通路。因此， [ 3 ] Farías M A, Diethelm-Varela B, Kalergis A M, et al. Interplay
糖基化的发生可能依赖于应激源与载体细胞的 between lipid metabolism, lipid droplets and RNA virus replica-
差异，而并非固定的蛋白修饰。 tion[J]. Critical Reviews in Microbiology, 2024, 50(5): 515-
综上，本研究通过对 poly(I:C) 刺激前后的 539.
草鱼头肾巨噬细胞蛋白进行 3 种修饰组学测定， [ 4 ] He L B, Wang Q, Liang X Y, et al. Grass carp reovirus induces
formation of lipid droplets as sites for its replication and
结果发现 poly(I:C) 可以调控蛋白泛素化，尤其
assembly[J]. mBio, 2022, 13(6): e0229722.
是诱导脂滴相关蛋白与磷脂代谢相关蛋白泛素
[ 5 ] Gaunt E R, Zhang Q F, Cheung W, et al. Lipidome analysis of
化，抑制脂肪酸 β 氧化关键酶的泛素化来改变
rotavirus-infected cells confirms the close interaction of lipid
脂质代谢与脂滴的合成。poly(I:C) 还可以诱导
droplets with viroplasms[J]. Journal of General Virology, 2013,
cPLA2 的磷酸化而调控磷脂的代谢。本实验揭
94(7): 1576-1586.
示了泛素化与磷酸化修饰在 poly(I:C) 诱导的脂
[ 6 ] Barbosa-Lima G, Hottz E D, De Assis E F, et al. Dengue virus-
质代谢中的重要作用。此外，本实验鉴定到差
activated platelets modulate monocyte immunometabolic
异蛋白的修饰位点及修饰肽段，为后续 dsRNA response through lipid droplet biogenesis and cytokine signal-
病毒调控巨噬细胞蛋白泛素化与磷酸化机制研 ing[J]. Journal of Leukocyte Biology, 2020, 108(4): 1293-1306.
究提供了参考。 [ 7 ] Zhou Y, Pu J, Wu Y P. The role of lipid metabolism in influ-

enza a virus infection[J]. Pathogens, 2021, 10(3): 303.
参考文献 (References)： [ 8 ] Salloum S, Wang H L, Ferguson C, et al. Rab18 binds to hepat-
[ 1 ] Olzmann J A, Carvalho P. Dynamics and functions of lipid itis C virus NS5A and promotes interaction between sites of
droplets[J]. Nature Reviews Molecular Cell Biology, 2019, viral replication and lipid droplets[J]. PLoS Pathogens, 2013,

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