Page 104 - 《水产学报》2025年第11期

李捷，等 水产学报, 2025, 49(11): 119608

Enzyme activities and gene expression in digestion and
metabolism of the triangle sail mussel (Hyriopsis cumingii) under
different temperature and diet ration

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LI Jie , SUN Yu , LIU Yang , YAO Weizhi , TENG Shujun , LI Yanhong , WU Zhengli 1
1. Research Center for Aquatic Biodiversity Conservation in the Upper Reaches of Yangtze River,
Ministry of Agriculture and Rural Affairs, College of Fisheries, Southwest University, Chongqing 400715, China;
2. Agricultural Science and Technology Extension Center of Tongnan District, Chongqing 402660, China
Abstract: The traditional culture method of Hyriopsis cumingii is in urgent need of transformation. Industrial aquaculture, a
sustainable cultivation mode, relies on the control of environmental conditions and food supply. To evaluate combined effects
of different rations (high-ration, medium-ration, and low-ration) and temperatures [(13.0±0.2), (23.0±0.2) and (33.0±0.2)℃)] on
digestion and metabolism of H. cumingii, a comprehensive 3 × 3 test factorial experiment was established. Digestive enzymes
(e.g., amylase, lipase, cellulase) and metabolic enzymes (e.g., glutamic pyruvate transaminase, glutamic oxaloacetate transam-
inase, pyruvate kinase, hexokinase) along with metabolism-related genes (e.g., CPT1, LDLR) were assayed over a 21-day
period in mussels. The results showed that levels of digestive and metabolic indicators remained stable in the medium-
ration×23℃ treatment, and were significantly higher than others on day 21 (P<0.05). When exposed to low or high-ration, as
well as 13 or 33℃, these biochemical indicators rose briefly and then significantly declined (P<0.05). Principal component ana-
lysis (PCA) demonstrated a clear separation of treatment groups based on digestive and hepatic enzyme activities, with PC1
accounting for 67.73%-80.73% of the total variance. Enzyme activities were notably reduced under extreme temperatures and
altered feeding levels, while lipase, amylase, cellulase, and key hepatic metabolic enzymes contributed positively to PC1.These
findings suggest that the medium-ration and a temperature of 23℃ were the optimal diet quantity and environmental conditions
for H. cumingii. Short-term thermal, cold, semi-starving, and overfeeding treatments induced metabolic levels of mussels to
meet the energy demand for anti-oxidative stress. However, long-term stress led to liver injury of H. cumingii, resulting in
reduced digestive and metabolic efficiency of the mussel, and subsequently affecting its growth and overall health. This study
holds practical significance in exploring culture environment and feeding conditions, controlling breeding cost, and increasing
growth and yield in industrial aquaculture.
Key words: Hyriopsis cumingii; temperature; ration level; digestion; metabolism
Corresponding author: LI Yanhong. E-mail: lyhong08@126.com
Funding projects: Chongqing Natural Science Foundation Project (CSTB2022NSCQ-MSX0305); China Agriculture Research
System (CARS-49); Chongqing Ecological Fisheries Industry Technology System (CQMAITS202315); Chongqing Key Fisher-
ies Science and Technology Project (CQFTIU2024-09)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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