Page 123 - 《水产学报》2025年第10期

李森栋，等 水产学报, 2025, 49(10): 109609

PDK1

PARP1 MAOA
PCNA

TOP1
IARS1
PRKDC

key target
potential target
active ingredients
center hub node
(a) (b)
pathway analysis
Toll-like receptor signaling pathway
Apoptosis count
5
NOD-like receptor signaling pathway 7
9
Adherens junction
11
Herpes simplex virus 1 infection 13
C-type lectin receptor signaling pathway pvalue
Base excision repair 0.000 20
0.000 15
ErbB signaling pathway 0.000 10
Focal adhesion 0.000 05
Progesterone-mediated oocyte maturation
4 5 6 7 8 9
enrichmentScore (−log 10 (pvalue))
(c) (d)
Fig. 5 Intersection analysis between network targets and transcriptomics, and related molecular binding analysis in
liver tissues of darkbarbel catfish.
(a) The circular network graph showcases the relationships between Key targets (ellipses), Active ingredients(diamonds), Potential targets (rectangles),
and Central hub node (triangles). (b) Docking of Withanolide A at the IARS1 receptor site with a docking score of -10.8. (c) PCNA-interacting proteins
among core target genes. (d) KEGG pathway enrichment analysis in core target.

byproduct of lipid peroxidation (Fig. 8-c), ROS, the responses and promotes the production of other
indicator of oxidative stress (Fig. 8-d), as well as cytokines [33-34] . IgM levels were significantly elev-
the expression level of heat shock protein 70 (HSP- ated in the basic dietary groups after being treated
70) (Fig. 8-e), while the dietary WS elevated the with both air exposure and physical stimulus (P <
SOD activity, an important antioxidative index (Fig. 0.05) (Fig. 9-a). In contrast, the WS dietary group
8-f).These data implied that WS supplementation exhibited a slight increase in IgM levels only afterr
could reduce the oxidative stress and protect liver air exposure, with no significant elevation observed
from damage during fish responding to any external under physical stimulus (Fig.9-a). Likewise, dietary
stimuli, such as injection, or air exposure. WS supplementation prominently suppressed the

Immune Factors modulated in fish Serum stress-induced elevation of inflammatory factors,
IgM in fish primarily mediates humoral immunity, including TNF-α (Fig. 9-b) and IL-6 (Fig. 9-c).
and its activation facilitates pathogen recognition, These data demonstrated that dietary WS interven-
neutralization, and maintenance of immune homeo- tion could alleviate stress-triggered immune
[32]
stasis . Elevated expression of TNF-α and inter- responses and maintain immune homeostasis in
leukin-6 (IL-6) is associated with inflammatory darkbarbel catfish.

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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