Page 125 - 《水产学报》2025年第10期

李森栋，等 水产学报, 2025, 49(10): 109609

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the proliferation of SG3 cells pomoted by WS-A DMSO
a
iars1 relative expression 10 bc b cells/mL 2.0×10 5 5 0.5 μmol/L
15
1 μmol/L
5 μmol/L
1.5×10
5
c 1.0×10 5
0 24 h 48 h 72 h
DMSO 0.5 μmol/L 1 μmol/L 5 μmol/L

(a) (b)
DMSO 0.5 μmol/L1 μmol/L 5 μmol/L
DMSO 0.5 μmol/L 1 μmol/L 5 μmol/L 100
PCNA
β-Tubulin PCNA a
(c) 90 b ab ab
2.5
relative expression 2.0 PCNA DAPI /DAPI ratio of PCNA positive cells/% 70
80
1.5
1.0
0.5
0
0.5 μmol/L
DMSO 1 μmol/L 5 μmol/L 60 DMSO 0.5 μmol/L 1 μmol/L 5 μmol/L

(d) (e) (f)
Fig. 7 The target genes’ expression and cells’ proliferation affected by Withanolide A treatment in SG3 cells
[30]
The SG3 cells (medaka fish spermatogonial cell line) were cultured as the previously reported , and plated in 96-well plates at 5 000 cells per well. Four
groups of cells were treated by 0.001% DMSO (the control), 0.5 μmol/L, 1 μmol/L and 5 μmol/L Withanolide A in medium respectively (n=5). (a) By
qPCR, iras mRNA expression was analyzed in cells after a 72-hour treatment of Withanolide A. (b) The analysis of cells’ proliferation using CCK-8 kit.
(c) After a 72-hour treatment of Withanolide A, The PCNA protein expression was determined in SG3 by Western blotting. (d) The relative expression
level of PCNA normalized by the level of housekeeping protein, β-Tubulin. (e) PCNA protein expression in cells was detected in SG3 via fluorescence
immunostaining. Scale bars, 100 μm. (f) The ratio of PCNA signal positive SG3 cells was examined, different letters indicate the significance of differences
(P < 0.05).
[45]
to stress. Especially, the Csf1a/Csf1rs signaling term detrimental effects on the fish’s health .
were reported to be a key regulator of embryonic Importantly, the transcriptome profiles of brain tis-
[43]
macrophage fates in zebrafish , theses suggested sue could be significantly altered during fish
that inflammatory response was induced by the responding to a physical stimuli, but the alterations
stress through upregulating the immune related of transcriptome profiles were prominently attenu-
genes, including the csf1a. Besides, grin1b, a sub- ated and the stress response were exclusively mitig-
unit of the NMDA receptor associated with a vari- ated by dietary WS in the darkbarbel catfish (NC vs.
ety of higher-order brain functions [44-45] , was also NP in Fig.2-b). Similarly, in rats, WS administra-
induced, implying some neuroadaptive response tion could significantly decrease alcohol paired
triggered by the stress in the darkbarbel catfish. The compartment alike Naltrexone through increasing
upregulation of these genes indicated that immune and reducing dopamine in the brain [46-47] . These find-
and neuronal activity are adaptively enhanced in ings indicated that WS supplementation may be
response to stress, although this heightened activity helpful for cognitive function and stress manage-
could potentially be maladaptive, leading to long- ment in animals, including in the fish species.

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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