Page 130 - 《水产学报》2025年第10期

李森栋，等 水产学报, 2025, 49(10): 109609

tion and then impair protein synthesis, thus result in a dietary supplement in aquaculture to solve prob-
[67]
mitochondrial dysfunction in mice . Watanabe et. lems of liver injuries and stress responses induced
al. reported that Withanolide J may antagonize by enviromental factors in fish species.

inflammation and oxidative stress by inhibiting the
4 Conclusion
NF-κB/STAT3 pathway, but its high doses may
cause hepatotoxicity due to the collapse of mito- This study reported that dietary WS could mit-
[68]
chondrial membrane potential . Furthermore, igate the stress response induced by physical stimu-
Withanolide A could induce ferroptosis via elevat- lus through inhibiting the alterations of transcrip-
ing ROS levels and inhibiting GPX4, offering novel tome profiles in brain tissues of darkbarbel catfish.
[69]
therapeutic strategies for liver diseases . Also, it was found that WS would majorly modulate
In summary, WS exerts hepatoprotection the oxidative stress and immune response pathways
through promoting hepatocyte proliferation, mitig- through the high binding affinities of its active com-
ating oxidative stress, and regulating homeostasis- ponents and related targets, including Withanolide A
related pathways in darkbarbel catfish, this would and Withanolide J binding with the active sites of
promote the applications of WS, even other herbs, as MAPK8 and IARS1 respectively (Fig. 12).

immune system

stress COR
liver
NF-κB Pathway
MAPK Pathway

Withanolide A
Withanolide D MAPK8
Withanolide J IARS1
Campesterol CRHR1 PI3K-Akt Pathway
Quercetin NR3C2
WS

Fig. 12 Molecular network of WS dietary acts on stress-relief and hepatic protection in darkbarbel catfish
Stress, injection or air exposure for 90 s. COR, cortisol. MAPK8 (Mitogen-activated protein kinase 8). IARS1 (Isoleucyl-tRNA synthetase 1). CRHR1
(Corticotropin releasing hormone receptor 1). NR3C2 (Nuclear receptor subfamily 3 group C member 2).

Likewise, the WS dietary could significantly mental data in aquatic animals.
allieviate fish stress response via suppressing the
COR, GLU, ROS, TNF-α and IL-6. More import- 参考文献 (References)：
antly, dietary WS prominently protected the liver [ 1 ] Abram Q, Dixon B, Katzenback B. Impacts of low temperature
health and promoted hepatocytic proliferation on the teleost immune system[J]. Biology, 2017, 6(4): 39.
through reducing the lipid accumulation, hepato- [ 2 ] Herrera M, Mancera J M, Costas B. The use of dietary addit-
cyte vacuolization and increasing hepatic PCNA ives in fish stress mitigation: comparative endocrine and
expression in darkbarbel catfish. Therefore, the physiological responses[J]. Frontiers in Endocrinology, 2019,
findings of this study provide new index to study 10: 47.
the mechanisms whereby the WS, even other herbs [ 3 ] Portz D E, Woodley C M, Cech J J. Stress-associated impacts of
act in stress-relief and liver protection via combin- short-term holding on fishes.[J]. Reviews in Fish Biology and
ing network pharmacological analysis and experi- Fisheries, 2006, 16(2): 125-170.

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