Page 126 - 《水产学报》2025年第10期

李森栋，等 水产学报, 2025, 49(10): 109609

8 4 4
a no stress ab a a ab
air exposure b
b physical stimulus 3 a b 3 c
6
COR/(ng/mL) 4 c d c c GLU/(μmol/mL) 2 1 d c d c MDA/(nmol/mL) 2
1
2
0 0 0
control Withania control Withania control Withania
somnifera somnifera somnifera
(a) (b) (c)
1 000 0.8 30
a
a a a ab ab ab a
800 b b 0.6 b c b bc 20 b ab
ROS/(pg/mL) 600 c c HSP-70/(ng/mL) 0.4 SOD/(U/mL) 10

400
0.2
200
0 0 0
control Withania control Withania control Withania
somnifera somnifera somnifera
(d) (e) (f)
Fig. 8 Stress indicators affected by dietary WS in adult darkbarbel catfish serum
The adult fish were fed with basic diet or WS for 5 weeks and subjected to different stress conditions for 90 second, including air exposure or physical
stimulus. And then the fish were sacrificed and the serum samples were collected for analysis.The groups of fish included the normal (no stress) as the
control, air exposure, and physical stimulus (injected with normal saline). (a) COR levels (ng/mL). (b) GLU levels (µmol/mL). (c) MDA levels
(nmol/mL), (d) ROS levels (pg/mL), (e) HSP-70 expression levels (ng/mL), and (f) SOD activity (U/mL). Different letters indicate the significant differ-
ences (P < 0.05), and the same letters for no significant difference.

6 000 a a 800 80 no stress
air exposure
b a a
bc c bc 600 a a 60 physical stimulus
IgM/(μg/mL) 2 000 TNF-α/(pg/mL) 400 bc c b b IL-6/(pg/mL) 40 c c b b
4 000
200
20
0 0 0
control Withania control Withania control Withania
somnifera somnifera somnifera
(a) (b) (c)
Fig. 9 Immune factors examined in adult darkbarbel catfish serum
The adult fish were fed with control diet or WS for 5 weeks and subjected to different stress conditions for 90 second, including air exposure or physical
stimulus. And then the fish were sacrificed and the serum samples were collected for analysis. The groups of fish included the normal (no stress) as the
control (white bar), air exposure (leaving water, light gray bar), and physical stimulus (injected with normal saline, dark gray bar). (a) IgM levels
(µg/mL), (b) TNF-α levels (pg/mL), and (c) IL-6 levels (pg/mL). Different letters indicate the significant differences (P < 0.05), and the same letters no
significant difference.

Recent studies demonstrated that the adapto- ults on WS were meta-analyzed, and it was found
genic herbs, including WS, are considered to have a that WS could significantly reduce serum COR and
stress-relieving effect and make animals adapt to decrease clinically relevant Perceived Stress Scale
[48]
environmental changes via reducing the COR levels (PSS) score after 56 or 60 days treatment . More
triggered by stress. Recent twenty-five studies on importantly, the previous studies demonstrated that
nine adaptogenic herbs, were sumarized. The res- the anti‑ inflammatory effect of WS probably was

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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