Page 122 - 《水产学报》2025年第10期

李森栋，等 水产学报, 2025, 49(10): 109609

(c)

key target
potential target
active ingredients
center hub node
(a)
pathway analysis
RIG-I-like receptor signaling pathway (d)
count
Toll-like receptor signaling pathway 1.00
1.25
Mitophagy-animal 1.50
1.75
NOD-like receptor signaling pathway 2.00
pvalue
Autophagy-animal
Hormone signaling 0.04
0.03
Cytosolic DNA-sensing pathway 0.02
0.01
Adipocytokine signaling pathway
1.5 2.0 2.5 3.0
enrichmentScore (−log 10 (pvalue))
(b) (e)
Fig. 4 Intersection analysis between network targets and transcriptomics, and related molecular binding analysis in
brain tissues of darkbarbel catfish.
(a) The circular network graph displays the relationships between Key targets (ellipses), Active ingredients(diamonds), Potential targets (rectangles), and
Central hub node (triangles). (b) KEGG pathway enrichment analysis in core target. (c) Docking of Withanolide A at the MAPK8 receptor site with a
docking score of -8.0. (d) Docking of Withanolide A at the CRFR1 receptor site with a docking score of -10.8. (e) Docking of Withanolide A at the
NR3C2 receptor site with a docking score of -9.8.

dosage maybe elevate cortisol level and promote 2.7 Serum Biomarkers Modulated by Stress
stress in fish. and/or dietary WS in adult darkbarbel catfish

2.6 Cell Proliferation and related genes’ The Biomarkers altered in Fish Serum by
expressions regulated by Withanolide A in fish Stress Cortisol (COR), was also found to be sig-
cells line nificantly elevated in both the traditional diet (con-

To confirm and illustrate functions of Withan- trol) and WS supplementation dietary (Withania som-
olide A in promoting the cells’ proliferation. SG3 nifera) groups, treated with air exposure or physical
cells were treated with DMSO (control) or 0.5, 1, or stimulus (P < 0.05) (Fig. 8-a). Notably, both the air
5 µmol/L Withanolide A, during being treated, the exposure and physical stimuli significantly increased
cells’ proliferation were analyzed at three time points, COR level in fish serum of the traditional dietary
24-, 48- and 72-h, the result showed that the iars1 groups, but slightly induced COR in those of the WS
mRNA expression was significantly increased by dietary groups (Fig. 8-a), suggesting that the supple-
Withanolide A treatment in SG3 cells (Fig. 7-a). mentation of WS may reduce the induction of COR
Also, the SG3 cells proliferation were significantly in fish serum during responding to stress.
triggered by Withanolide A (Fig. 7-b). Moreover, Similarly, the WS dietary significantly
the PCNA protein was significantly upregulated in decreased levels of the GLU, representing meta-
SG3 cells treated with Withanolide A (Fig. 7-c—f). bolic adaptations to stress (Fig. 8-b), MDA, a

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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