Page 119 - 《水产学报》2025年第10期

李森栋，等 水产学报, 2025, 49(10): 109609

20
CP vs. CC NP vs. NC
down down
normal normal
15 up zgc-122979 up
15
downregulated: 785 downregulated: 255
upregulated: 666 upregulated: 124
−Log 10 FDR 10 npas4a −Log 10 FDR 10

casq2
5 cacna1ab
5
grin1b CYTB
wnk1b
csf1a
0 0
−20 −10 0 10 20 0 10 20 30
Log 2 FC Log 2 FC
(a) (b)
500 5
up down up down
400
CC 4
300
CP
200 3
FPKM 100 mRNA relative expression
50
40 2
30
20 1
10
0 0
cacna1ab casq2 csf1a grin1b npas4a wnk1b oxt foxg1a dlx6a nkx2.1 npvf cacna1ab casq2 csf1a grin1b npas4a wnk1b oxt foxg1a dlx6a nkx2.1 npvf

(c) (d)
Fig. 2 Differential expressed genes (DEGs) analysis and validation by qPCR in darkbarbel catfish brain
The adult fish were fed with WS for 5 weeks and subjected to physical stimulus for 90 seconds. And then the 6 individuals of each group were sacrificed
and the brain samples were collected for the RNA-seqs analysis. The groups of fish included the CC (no stress, no WS supplementation), the CP (subjec-
ted to physical stimulus (injected with normal saline), no WS supplementation), the NP (subjected to physical stimulus (injected with normal saline), WS
supplementation), the NC (no stress, WS supplementation). (a) CP vs. CC. (b) NP vs. NC. The selected DEGs (CP vs. CC) of RNA-seq dataset (c) valid-
ated by qPCR (d). Different letters indicate the significant differences (P < 0.05), and the same letters for no significant difference.

way, emphasizing its critical role in neuronal signal- pounds in WS with significant therapeutic potential.
ing and survival under stress, and other metabolic Applying the criteria of Drug Likeness (DL) ≥
pathways such as glycine, serine, and threonine 0.18 and Oral Bioavailability (OB) ≥ 30%, 13 bio-
metabolism, folate biosynthesis, as well as trypto- logically active compounds were identified (Tab.1).
phan and tyrosine metabolism (Fig. 3-c).
The “WS-ingredient-target” regulatory network was

2.3 Active ingredients and potential targets of constructed using Cytoscape software (Fig. 4-a).
WS in fish brain These compounds were further analyzed using vari-
Recent advancements in phytochemistry and ous biological databases, yielding 379 potential tar-
pharmacology facilitate identifying bioactive com- gets, which were refined by excluding duplicates.

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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