Page 114 - 《水产学报》2025年第10期

李森栋，等 水产学报, 2025, 49(10): 109609

feed supplement brain tissue

darkbarbel catfish
Withania somnifera next-generation sequencing
active
ingredients DEGs
kaempferol quercetin
OH OH
HO O O O 20 NP vs NC CP vs CC
OH down 15 down
normal
normal
OH 15 up up
OH molecular
OH O OHO downregulated: 255 downregulated: 785
docking −Log 10 FDR 10 upregulated: 124 −Log 10 FDR 10 upregulated: 666
Fucosterol 5 5
0 0
H 0 10 20 30 −20 −10 0 10 20
Log 2 FC Log 2 FC
H H
HO enrichment analysis
targets molecular docking GO Enrichment (CP vs CC)
neuropeptide signaling pathway
blood coagulation
regulation of body fluid levels Calcium signaling pathway pathway analysis
synaptic signaling count
trans-synaptic signaling 5
regulation of transmembrane transport count Glycine, serine and 10
behavior threonine metabolism
response to wounding 10 Vascular smooth muscle 15
10
15
GO Term somatodendritic compartment 20 Folate biosynthesis pvalue
inorganic ion homeostasis
chemical homeostasis
contraction
25
neuronal cell body
0.04
ion channel complex
cation channel complex ONTOLOGY 0.03
0.02
receptor complex Tryptophan metabolism
peptide receptor activity BP 0.01
hormone activity CC
signaling receptor regulator activity Tyrosine metabolism
channel activity MF
monoatomic cation channel activity 2 3 4
2 4 6
−log 10 (P-adjust) enrichmentScore [−log 10 (P-value)]
validation test 2
5 3
a a
a
COR/(ng/mL) 2 GLU/(μmol/mL) 1 b
4
validation test 1 3 b 2 b
0 1 0
no air physical no air physical
stressexposure stimulus stressexposure stimulus
800 30
a a a a a
ROS/(pg/mL) 400 b SOD/(U/mL) 20
600
10
200
data validated or confirmed by intramuscular injection of Withanolide A 0 no air physical 0 no air physical
in juvenile darkbarbel catfish, and in SG3 cells treated by Withanolide A stressexposure stimulus stressexposure stimulus
serum biochemical index
immune
system
stress COR liver
the illustrations of moleeular network
whereby Withania somnifera on stress-relief NF-κB Pathway
and hepatic protection in fish species MAPK Pathway
Withanolide A
Withanolide D MAPK8
Withanolide J IARS1
Campesterol CRHR1 PI3K-Akt Pathway
Quercetin NR3C2
WS
Fig. 1 The flowchart of experiments in this study
groups were maintained in flow-through tanks and prior to experimentation. Animal handling proto-
acclimated for 3 days under laboratory conditions cols were approved by the Animal Welfare Ethics
https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
4