Page 128 - 《水产学报》2025年第10期

李森栋，等 水产学报, 2025, 49(10): 109609

PCNA DAPI PCNA/DAPI

(a) CC

(b) NC

40 a
ratio of PCNA positive cells/% 20 b

30

10

0
CC NC
(c)
Fig. 11 The cells’ proliferation potency of liver affected by dietary WS in darkbarbel catfish
Liver cryo-sections were stained with the poly-clonal anti‐PCNA antibody (Red), the nuclei of cells were stained with 4',6-diamidino-2-phenylindole
(DAPI) (blue). (a) The groups of fish included the CC (no stress, no WS supplementation); (b) The NC (no stress, WS supplementation). Scale bars,
50 μm; (c) The ratio of PCNA positive cells in liver tissues, different letters indicate the significance of differences (P < 0.05).

employs a network structure of “multicomponent- that active phytochemicals from herbs could poten-
multitarget” and allows comprehensive investiga- tially inhibit SARS-CoV-2 Mpro and further equip
[13]
tions on drug-illness interactions . For example, the management strategy against COVID-19 a
[56]
groups have find out the bioactive components to global contagion .
treat Autism Spectrum Disorder (ASD) based on the Therefore, network pharmacology is a power-
[13]
analysis of a network pharmacology . Precise and ful tool for identifying the molecular targets of bio-
effective therapeutic intervention is achieved via active compounds and offers valuable insights into
network pharmacology and drug reusing, thus understandings on mechanisms of WS actions on
speeding up clinical translation [26,55] . Intriguingly, disorders in organisms. In this study, network phar-
the molecular docking analysis of network pharma- macological analysis were conducted to explore the
cology figured out the two potential inhibitors potential targets and molecular pathways associated
against SARS-CoV-2 Mpro (Main protease) from with WS in alleviating stress in darkbarbel catfish.
WS [Withanoside V (−10.32 kcal/mol) and Somni- Firstly, the major active compounds of WS were
ferine (−9.62 kcal/mol)], and the docked complexes analyzed using literature-based databases, includ-
of active compounds-targets were proved to be con- ing Withanolide A, Withanolide D, and Withanolide
stant and stable in the further molecular dynamics J. Then the potential molecular targets of these
(MD) simulation study. Hence it could be deduced compounds were analyzed, including pathways

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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