Page 79 - 《中国药科大学学报》2025年第4期

第 56 卷第 4 期 郭光耀，等：PPARβ 激动剂 ZLY16 促进肌再生及改善 mdx 小鼠运动能力 475

A WT Model ZLY16 Simvastatin

TA
DAPI
MHC
eMyHC

GAS
DAPI
MHC
eMyHC

DIA
DAPI
MHC
eMyHC

B TA C GAS D DIA
20 ** 10 8 ** 6 *
Area of eMyHC/% 10 ** Area of eMyHC/% 6 4 ** Area of eMyHC/% 4 2
15

5

0 2 0 0
WT Model ZLY16 Simvastatin WT Model ZLY16 Simvastatin WT Model ZLY16 Simvastatin

E F PPARβ G CPT-1a H Myogenin
WT Model ZLY16 Simvastatin 1.5 ** 1.5 ** 6 *
CPT-1a 88 kD 1.0 ## ** 1.0 ** 4

PPARβ 52 kD Relative protein expression 0.5 Relative protein expression 0.5 ## Relative protein expression 2
Myogenin 34 kD

GAPDH 36 kD 0 WT Model ZLY16 Simvastatin 0 WT Model ZLY16 Simvastatin 0 WT Model ZLY16 Simvastatin

Figure 7 ZLY16 promoted skeletal muscle regeneration in mdx mice（ x ± s, n=6）
A： TA, GAS and DIA were stained with eMyHC (green), MHC (red) and DAPI (blue), eMyHC represents new muscle fibers, MHC
represents mature muscle fibers and DAPI represents nuclei. (scale bar: 50 μm); B: Percentage of eMyHC area were qualified in TA from
mdx mice; C: Percentage of eMyHC area were qualified in GAS from mdx mice; D: Percentage of eMyHC area were qualified in DIA from
mdx mice. E: Western blotting of PPARβ, CPT-1a and myogenin protein levels in mdx mice; F: Quantification of PPARβ protein levels in
mdx mice; G: Quantification of CPT-1a protein levels in mdx mice; H: Quantification of myogenin protein levels in mdx mice
##
P < 0.01 vs WT group; *P < 0.05, **P < 0.01 vs model group
细胞活性（P<0.01）。 所示，相对于空白组，PA 组显著增加了成肌细胞内
使用马血清诱导高脂环境中 C2C12 细胞分 的脂质，抑制成肌细胞分化为肌管。相较于 PA 组，

化，同时分别给予不同浓度的 ZLY16（0、5、10、20 给药 ZLY16 可以明显减少成肌细胞周围的脂质蓄
μmol/L） 和 10 μmol/L 的 simvastatin， 考 察 ZLY16 积，同时促进成肌细胞的分化和肌管融合。此外，
对成肌细胞分化是否有促进作用。结果如图 8-B 蛋白免疫印迹实验结果如图 8-C–8-F 所示，ZLY16