Page 107 - 《中国药科大学学报》2025年第4期

第 56 卷第 4 期 曲 晶，等：肾上腺素 β 受体阻断药对小鼠睡眠结构的影响 503
A Vehicle Metoprolol

day1 day2
6 h calculation 6 h calculation
B 150 * * E 100
Total wakefulness time/min 100 Wakefulness bouts 60
80

40
50
20

0
5 10 20 40 80 0 5 10 20 40 80
Dosage/(mg/kg) Dosage/(mg/kg)
C 250 * F 80
Total NREMS time/min 150 NREMS bouts 60
200

40
100
20
50
0 0
5 10 20 40 80 5 10 20 40 80
Dosage/(mg/kg) Dosage/(mg/kg)
D 50 ** G 60
** ** ** * ** **
Total REMS time/min 30 REMS bouts 40
40

20
20
10
0 0
5 10 20 40 80 5 10 20 40 80
Dosage/(mg/kg) Dosage/(mg/kg)
H 150

Micro arousal No. 100 Vehicle_Adrb1 ; +/+
+/+
Metoprolol_Adrb1 ;
Vehicle_Adrb1 ;
+/m
50
Metoprolol_Adrb1
+/m
0
5 10 20 40 80
Dosage/(mg/kg)

Figure 2 Intraperitoneal metoprolol promotes wakefulness with 6 hours. (A) Schematic workflow of experiment setup. (B-H) The
sleep/wake status of wild-type mice (n=11 for all dosages) and mutant mice (n=9,5mg/kg; n=7,10mg/kg; n=10,20mg/kg; n=8,40mg/kg;
n=10,80mg/kg) within 6 hours after administration were calculated for wakefulness duration (B), NREMS duration (C) , REMS duration
(D), wakefulness bouts (E), NREMS bouts (F) ,REMS bouts (G) and micro arousal numbers (H) . *P<0.05, **P<0.01. Two-way ANOVA,
post-hoc Sidak's multiple comparisons test. Three-way ANOVA to compare the difference between genotypes
具有抑制作用。另一方面，醋丁洛尔对睡眠指标 剂量。考虑到临床推荐的人体日剂量为普萘洛尔
的影响微乎其微（图 5-N~5-S）。值得一提的是，在 30 mg，比索洛尔 5 mg，醋丁洛尔 400 mg，因而本
本研究中，所有 3 种药物均采用 10 mg/kg 的统一 研究观察到的醋丁洛尔缺乏效果可能是由于剂量