Page 98 - 《渔业研究》2026年第1期

第 1 期 牟 桐等： 基于高分辨液相色谱与质谱联用系统分析银鲳肌肉脂质组成 95

Analysis of lipid composition in Pampus argenteus muscle using high-resolution
liquid chromatography-mass spectrometry

1
1
1*
1
MU Tong ，HUANG Lili ，ZHU Zhujun ，LI Yanrong ，XU Jilin 2
(1. Ningbo Institute of Oceanography, Ningbo 315832, China;
2. School of Marine Sciences, Ningbo University, Ningbo 315211, China)
Abstract: [Objective] This study aims to elucidate the lipid composition of silver pomfret (Pampus argenteus)
muscle, establishing a scientific foundation for its potential applications in food processing and functional food
development. [Methods] Wild P. argenteus samples were freeze-dried and ground into powder using liquid ni-
trogen. Total lipids were extracted from the muscle samples using methanol and chloroform. The lipid profile in
muscle of P. argenteus was explored by high-resolution liquid chromatography-mass spectrometry equipped
with a HESI-Ⅱ probe. [Results] A total of 447 lipids belonging to 17 major classes were identified in P. ar-
genteus. These included 4 classes of sphingolipids: sphingosine (So), ceramide (Cer), monoglycosylceramide
(CerG1), and sphingomyelin (SM); 2 classes of neutral glycerolipids: diacylglycerol (DAG) and triacylglycerol
(TAG); 11 classes of phospholipids: lysophosphatidylcholine (LPC), phosphatidylcholine (PC), lysophos-
phatidy- lethanolamine (LPE), phosphatidylethanolamine (PE), lysophosphatidylserine (LPS), phosphatidylser-
ine (PS), lysophosphatidylglycerol (LPG), phosphatidylglycerol (PG), lysophosphatidylinositol (LPI), phos-
phatidylinositol (PI), and phosphatidic acid (PA). Quantitative analysis demonstrated that neutral glycerolipids
(54.93%) and phospholipids (43.82%) constituted the predominant lipid fractions. Triglycerides (TAG) repres-
ented the most abundant lipid species (38.37% of total lipids), while phosphatidylcholine (25.32%) and phos-
phatidylethanolamine (11.48%) emerged as the major phospholipid components. Fatty acid profiling identified
32 molecular species, comprising 11 saturated fatty acids (SFA), 8 monounsaturated fatty acids (MUFA), and 13
polyunsaturated fatty acids (PUFA), with PUFA exhibiting marginally higher abundance than SFA and MUFA.
The major fatty acids in P. argenteus muscle were palmitic acid (C16:0), oleic acid (C18:1), eicosapentaenoic
acid (EPA), and docosahexaenoic acid (DHA). [Conclution] High-resolution liquid chromatography-mass
spectrometry analysis demonstrated that P. argenteus muscle contains a diverse array of lipids, with notably
high levels of DHA and EPA, supporting its classification as a nutritious seafood under the “Blue Granary”
framework.
Key words: Pampus argenteus; lipids; fatty acids; high-resolution liquid chromatography-mass spectrometry