Page 142 - 《渔业研究》2025年第6期

第 6 期 王 峥等： 饲料蛋白水平对瓦氏雅罗鱼生长性能、肌肉品质、消化酶及非特异性免疫的影响 833

Effects of different dietary protein levels on growth performance, muscle
quality, digestive enzymes and non-specific immunity of Leuciscus waleckii

WANG Zheng，LIU Yixin，YU Jiuxiang，WANG Lei，JIN Guanghai *
(Liaoning Key Laboratory of Aquatic Animal Diseases Control, Liaoning Institute of Freshwater Fisheries, Liaoyang 111000, China)

Abstract: [Objective] This experiment aims to investigate the relationship between dietary protein levels and
growth performance, muscle quality, and biochemical parameters in Amur ide (Leuciscus waleckii), and further
determine the optimal dietary protein level for its growth conditions. [Methods] In the experiment, 600 healthy
L. waleckii with an average initial body mass of (49.42±5.39) g were selected and randomly divided into five
groups, with three replicates in each group and 40 fish in each replicate. The crude protein content of each group
was respectively 25.68%, 29.84%, 34.52%, 39.04%, 43.12% of five groups of diets with the same fat content.
The experiment period was 56 days. The growth performance indicators including survival rate (SR), weight
gain rate (WGR), specific growth rate (SGR), feed conversion ratio (FCR), and condition factor (CF) of the ex-
perimental fish, as well as the proximate composition of muscle and relevant enzyme activities in the liver and
intestine were detected. [Results] The results showed that the weight gain rate and specific growth rate of test
fish in the 34.52% group were significantly higher than those in the 25.68% group and the 29.84% group
(P<0.05). The feed conversion ratio of test fish in the 43.12% group was significantly lower than that of the
25.68% group and the 29.84% group (P<0.05). The crude protein content of test fish in the 34.52% group was
significantly higher than that of the 25.68% group, the 29.84% group and the 43.12% group (P<0.05). The crude
fat content of test fish in the 43.12% group was significantly higher than that of the 29.84% group and the
34.52% group (P<0.05). The amylase activity in the intestinal tract of test fish in the 34.52% group was signific-
antly higher than that in the 25.68% group, the 29.84% group and the 43.12% group (P<0.05). The lipase activ-
ity in the intestinal tract of test fish in the 34.52% group was significantly higher than that in the other groups
(P<0.05). The protease activity in the intestinal tract of test fish in the 43.12% group was significantly higher
than that in the 25.68% group, the 29.84% group and the 34.52% group (P<0.05). The acid phosphatase of test
fish in the 34.52% group was significantly higher than that in the 25.68% group (P<0.05). [Conclusion] The
results show that through polynomial regression analysis of the relationship between the weight gain rate and the
crude protein level in the dietary, it is concluded that the most suitable dietary protein level for the sub-adult
growth stage of L. waleckii is 37.52%.
Key words: Leuciscus waleckii ; dietary protein level; growth performance; muscle quality; biochemical indices