Page 77 - 《渔业研究》2025年第5期

618 渔 业 研 究 第 47 卷

学报，2013，37（3） ：450 − 456. 业，2018，48（6） ：9 − 16.
Zeng W W, Wang Q, Wang Y Y, et al. Preparation and Fan Y D, Ma J, Zhou Y, et al. Establishment and applic-
identification of monoclonal antibody against VP4 pro- ation of a universal RT-PCR assay for detection of dif-
tein of grass carp reovirus HZ08 strain[J]. Journal of ferent genotype grass carp reovirus[J]. Freshwater Fish-
Fisheries of China, 2013, 37(3): 450 − 456. eries, 2018, 48(6): 9 − 16.
［13］ Wu H L, Yin J Y, Li S M, et al. Oral immunization with ［18］ Yan L M, Guo H, Sun X Y, et al. Characterization of
recombinant L. lactis expressing GCRV-Ⅱ VP4 produ- grass carp reovirus minor core protein VP4[J]. Virology
ces protection against grass carp reovirus infection[J]. Journal, 2012, 9: 89.
Fish & Shellfish Immunology, 2024, 147: 109439. ［19］ Qiu D K, Xiong Y, Gong Y M, et al. Dominant antigen
［14］ Rao Y L, Su J G. Insights into the antiviral immunity of grass carp reovirus and immunity assessment with
against grass carp (Ctenopharyngodon idella) reovirus DNA vaccine for grass carp[J]. Aquaculture, 2021, 530:
(GCRV) in grass carp[J]. Journal of Immunology Re- 735948.
search, 2015, 2015: 670437. ［20］ González-Montalbán N, García-Fruitós E, Villaverde A.
［15］ Zhang L L, Luo Q, Fang Q, et al. An improved RT-PCR Recombinant protein solubility—does more mean bet-
assay for rapid and sensitive detection of grass carp re- ter?[J]. Nature Biotechnology, 2007, 25(7): 718 − 720.
ovirus[J]. Journal of Virological Methods, 2010, 169(1): ［21］ Singhvi P, Saneja A, Srichandan S, et al. Bacterial inclu-
28 − 33. sion bodies: a treasure trove of bioactive proteins[J].
［16］ 曾伟伟，王庆，王英英，等. 草鱼呼肠孤病毒三重 Trends in Biotechnology, 2020, 38(5): 474 − 486.
PCR 检测方法的建立及其应用 [J]. 中国水产科学， ［22］ Peternel Š, Bele M, Gaberc-Porekar V, et al. Nonclassical
2013，20（2） ：419 − 426. inclusion bodies in Escherichia coli[J]. Microbial Cell
Zeng W W, Wang Q, Wang Y Y, et al. Establishment of Factories, 2006, 5(1): P23.
multiplex PCR for detection of grass carp reovirus and ［23］ 迟象阳，于长明，陈薇. 单个 B 细胞抗体制备技术及
its application[J]. Journal of Fishery Sciences of China, 应用 [J]. 生物工程学报，2012，28（6） ：651 − 660.
2013, 20(2): 419 − 426. Chi X Y, Yu C M, Chen W. Single B cell monoclonal
［17］ 范玉顶，马杰，周勇，等. 不同基因型草鱼呼肠孤病 antibody technologies and applications[J]. Chinese Jour-
毒通用 RT-PCR 检测方法的建立及应用 [J]. 淡水渔 nal of Biotechnology, 2012, 28(6): 651 − 660.