Page 186 - 《水产学报》2026年第3期

3 期 张天华，等：饲料中添加不同水平纳米氧化铁对黄颡鱼幼鱼肌肉发育的影响及其机理 50 卷

Effects and mechanism of different levels of Fe O nanoparticles on
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3
muscle development in juvenile yellow catfish (Pelteobagrus fulvidraco)

ZHANG Tianhua , WEI Xiaolei , GUO Jiacheng , LIU Lulu , DUAN Fuxuan , LUO Zhi *
(Hubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan 430070, China)

Abstract: To investigate the effects of different levels of nano-iron oxide on the muscle development of young yellow catfish
(Pelteobagrus fulvidraco), using Fe 2 O 3 NPs as the Fe sources, the present study formulated three experimental diets with diet-
ary Fe levels at 22.23 (control), 44.53 (Low Fe 2 O 3 NPs group), 61.85 mg Fe/kg diet (High Fe 2 O 3 NPs group), respectively, and
fed to P. fulvidraco for 10 weeks. Our aims were to investigate whether and how different levels of Fe 2 O 3 NPs affected the
muscle development of juvenile P. fulvidraco. Low dietary Fe 2 O 3 NPs level significantly augmented muscle fiber diameter,
stimulated protein deposition, decreased NEFA and MDA levels in muscle tissue, elevated GSH content, upregulated the
mRNA expression of MRF family members and fsp1 and slc7a11 genes, downregulated the mRNA expression of mstn gene
and inhibited both mRNA and protein expressions of inflammatory factor nf-κb1 gene. The high Fe 2 O 3 NPs diets inhibited
muscle fiber diameter, increased the contents of iron, NEFA and MDA, induced GSH depletion, stimulated the expression of
ferroptosis related genes and inhibited the expression of fsp1 and slc7a11 genes, and increased mRNA and protein expression of
inflammatory factor nf-κb1 gene. Moreover, we cloned the sequences of myog and slc7a11 promoters from yellow catfish, and
predicted the potential binding sites for NF-κB1 transcription factor on these two promoters. The dual luciferase activity assay
and EMSA indicated that the NF-κB1 transcription factor directly binds to the myog promoter (-6 bp/-16 bp) and the slc7a11
promoter (-430 bp/-439 bp). Furthermore, NF-κB1 transcription factor plays a direct role in mediating the transcription and reg-
ulation of these promoters; Fe 2 O 3 NPs incubation enhanced this binding. This study revealed the potential mechanism of the
regulation of muscle development by Fe 2 O 3 NPs, and provided a good basis for dietary Fe 2 O 3 NPs addition in the aquafeeds.

Key words: Pelteobagrus fulvidraco; Fe 2 O 3 NPs; muscle development; transcription and regulation
Corresponding author: LUO Zhi. E-mail: luozhi99@mail.hzau.edu.cn
Funding projects: National Natural Science Foundation of China (32220103013); National Key R & D Program of China
(2023YFD2400600)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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