Page 48 - 《水产学报》2025年第10期

孙倩文，等 水产学报, 2025, 49(10): 109603

Effects of different levels of methionine and taurine in
all-plant-protein diets on growth, physiology and antioxidant capacity of
juvenile Megalobrama amblycephala

1,2
2
2
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SUN Qianwen , JIANG Wenqiang , LIN Yan , LU Siyue ,
2
GU Zhengyan , MIAO Linghong 1,2*
1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;
2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs,
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

Abstract: To explore the effect of supplementing methionine (Met) and taurine (Tau) in the all-plant-protein diet of juvenile
Megalobrama amblycephala, six experimental diets were formulated with three Met supplementary levels (2.5, 5.0 and 10.0
g/kg) and two Tau supplementary levels (1.0 and 2.0 g/kg), yielding measured concentrations of: M 1 T 1 (Met 7.5 g/kg and Tau
1.7 g/kg), M 1 T 2 (Met 7.2 g/kg and Tau 2.7 g/kg), M 2 T 1 (Met 7.9 g/kg and Tau 1.7 g/kg), M 2 T 2 (Met 8.6 g/kg and Tau 2.7 g/kg),
M 3 T 1 (Met 11.8 g/kg and Tau 1.6 g/kg) and M 3 T 2 (Met 12.5 g/kg and Tau 2.8 g/kg). After a 10-week feeding trial, growth per-
formance, plasma indices, and hepatic and intestinal antioxidant capacity were evaluated. Weight gain rate (WGR), specific
growth rate (SGR), and feed conversion ratio (FCR) were affected by dietary Met and by Met × Tau interaction; the M 2 T 2
group exhibited the highest WGR and SGR and the lowest FCR. Plasma alkaline phosphatase (ALP) was influenced by Met
and the Met × Tau interaction, whereas plasma alanine aminotransferase (ALT) was affected by Tau. At Tau level T 1 , ALP and
ALT declined as Met increased. Hepatic and intestinal catalase (CAT) activity was highest in M 2 T 2 and was influenced by the
Met × Tau interaction. Intestinal glutathione (GSH) responded to Met and Tau, whereas malondialdehyde (MDA) content was
affected by the interaction. At Tau level T 2 , GSH and MDA decreased with increasing Met. Intestinal amylase (AMS) and tryp-
sin (TPS) activities were affected by the Met × Tau interaction; at Tau level T 1 , TPS peaked in the M 2 T 1 group. Dietary inclu-
sion of Met 8.6 g/kg and Tau 2.7 g/kg in an all-plant-protein diet significantly improved growth, feed utilization and antioxid-
ant status, indicating a synergistic benefit of optimal Met and Tau supplementation.
Key words: Megalobrama amblycephala; all-plant-protein diet; methionine; taurine; growth performance; antioxidant capacity
Corresponding author: MIAO Linghong. E-mail: miaolh@ffrc.cn

Funding projects: China Agriculture Research System (CARS-45); Science and Technology Innovation Team (2023TD63)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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