Page 216 - 《水产学报》2025年第10期

郝甜甜，等 水产学报, 2025, 49(10): 109615

Effects of dietary selenium supplementation in high-plant-protein diets on
the growth performance, body composition, PPAR gene expression and
intestinal inflammatory response of juvenile turbot

(Scophthalmus maximus)

1
1
2
1,3
1
HAO Tiantian , LI Baoshan , YU Chaolei , HUANG Bingshan , LI Qian ,
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1,3
WANG Peifeng , SUN Yongzhi , WANG Jiying 1*
1. Key laboratory of Marine Ecological Restoration, Shandong Marine Fishery Feed Engineering Technology Research
Center , Shandong Marine Resources and Envionment Research Institute, Yantai 264006, China;
2. Yantai Jinghai Marine Fishery Co., Ltd., Yantai 264003, China;
3. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Centre for Research on
Environmental Ecology and Fish Nutrion of the Ministry of Agriculture and Rural Affairs, National Demonstration Center for
Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China

Abstract: Plant proteins have been increasingly used as sustainable substitutes for fishmeal in aquafeeds; however, their high
inclusion level compromises fish performance. Selenium (Se) is an essential micronutrient required for normal development and
antioxidant protection, but its effects on the growth, physiological metabolism, and immunity of marine fish in response to high-
plant-protein diets need further research. To investigate the effects of sodium selenite on growth performance, body composi-
tion, ppars gene expression, and intestinal inflammatory response of juvenile turbot fed off a high-plant-protein diet. A total of
525 turbot [(30.79±0.22) g] were randomly divided into 7 groups (3 replicates each): DP, DN, D1, D2, D3, D4 and D5. After a
52-day feeding trial, sodium selenite supplementation alleviated growth inhibition caused by the high-plant-protein diet,
improving weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER), and condition factor (CF). Feed
coefficient showed the opposite trend and was significantly higher in the DN group than in others. In whole-body composition,
crude lipid content in whole fish and dorsal muscle was significantly reduced in the DN group, whereas hepatic crude lipid con-
tent was significantly increased. Appropriate sodium selenite relieved hepatic lipid accumulation caused by low fishmeal. Addi-
tionally, sodium selenite mitigated the reduction in serum activities (total cholesterol, lysozyme, alkaline phosphatase, and total
superoxide dismutase) induced by the high-plant-protein diet. Appropriate sodium selenite inhibited the decrease of pparα1,
pparα2, and pparβ mRNA levels and the increase of pparγ mRNA levels induced by the high-plant-protein diet. Sodium selen-
ite also increased TGF-β1 content and decreased TNF-α and IL-6 contents in the intestinal. In conclusion, sodium selenite mit-
igated the negative effects of the high-plant-protein diets on growth performance, hepatic lipid deposition, immunity, and intest-
inal health; the recommended supplemental level is 0.6–2.4 mg/kg. This study lays a theoretical foundation for further explora-
tion of sodium selenite in aquatic formulated feeds.
Key words: Scophthalmus maximus; selenium; high-plant-protein diet; growth performance; body composition; ppars; inflam-
matory factor

Corresponding author: WANG Jiying. E-mail: ytwjy@126.com
Funding projects: Shandong Provincial Natural Science Foundation(ZR2020QC205)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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