Page 153 - 《水产学报》2025年第10期

陈强，等 水产学报, 2025, 49(10): 109610

DHA-rich marine phosphatidylcholine alleviates lipid deposition and
mediates phospholipid remodeling in
largemouth bass (Micropterus salmoides)

1*
2
1
1
1
CHEN Qiang , ZHANG Runzhe , REN Tianli , QIAN Yuanxin , TANG Yunping ,
1
1
DONG Fen , WANG Jiteng , HAN Tao 1*
1. Fisheries College, Zhejiang Ocean University, Zhoushan 316022, China;
2. Food and Pharmacy College, Zhejiang Ocean University, Zhoushan 316022, China
Abstract: This study investigated the effects of DHA-rich phosphatidylcholine (DHA-PC) extracted from Atlantic herring
(Clupea harengus) eggs on growth performance, fat metabolism, and lipid composition of largemouth bass (Micropterus sal-
moides). Three isonitrogenous (48% crude protein) and isolipidic (12% crude fat) diets were prepared, containing 0, 3% and 6%
DHA-PC, designated CON, MDHAPC and HDHAPC. The largemouth bass with initial weight of (4.31 ± 0. 038) g were fed the
diets for 8 weeks. DHA-PC supplementation did not affect growth performance but significantly reduced the viscera-somatic
index (VSI). Crude fat content in whole body decreased significantly with DHA-PC inclusion. The analysis of fatty acid com-
position showed that DHA-PC had a significant effect on improving the contents of DHA and EPA in fish, serum and muscle.
In terms of physiological and biochemical indexes related to lipid metabolism, DHA-PC significantly reduced the content of
triglyceride (TG) in serum, liver and muscle; DHA-PC significantly reduced the mRNA expression levels of genes related to fat
synthesis (acc1 and fas, etc.) in liver and muscle, but significantly increased the mRNA expression levels of genes related to
lipolysis (lpl) and fatty acid oxidation (cpt1). Muscle tissue lipidomics analysis found that the addition of DHA-PC altered the
distribution of fatty acids in PC, PE (phosphatidylethanolamine), and PI (phosphatidylinositol). Analyzing the expression of
genes related to phospholipid synthesis, it was found that DHA-PC significantly promoted the mRNA expression levels of cct-
α, cctβ and pemt. DHA-PC had no significant effect on the growth performance of largemouth bass, and significantly reduced
fat levels within tissues by inhibiting fat synthesis and promoting fatty acid oxidation and lipolysis. DHA-PC extracted from
aquatic by-products is a good lipid-lowering feed additive for largemouth bass while maximizing the utilization of marine
resources.
Key words: Micropterus salmoides; DHA-PC; growth performance; lipidomics; fat metabolism

Corresponding authors: CHEN Qiang. E-mail address: chenqiang@zjou.edu.cn;
HAN Tao. E-mail address: goodhantao@gmail.com

Funding projects: National Natural Science Foundation of China (32303023); Zhejiang Provincial Natural Science Founda-
tion of China under Grant (LQ24C190006) ; Fundamental Research Funds for Zhejiang Provincial Universities and Research
Institutes ( 2024J004–3).

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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