Page 191 - 《水产学报》2025年第8期

曹磊，等 水产学报, 2025, 49(8): 089416

1 500
10 1 000 **** ****
log 2 (唯一肽段数+1) log 2 (no. of unique peptide+1) 6 泛醌氧化还 β半乳糖苷酶活性 β-galactosidase activity 500
8

原酶亚基
4
A7NDUFA7
2

−1
−3
−2
0
不同实验组
log (比值) 1 2 3 0 1 2 3
2
log (ratio) different experimental groups
2
(a) (b)
脂质代谢 lipid metabolism 6
全局概览图谱 global and overview maps 3
碳水化合物代谢 carbohydrate metabolism 3
辅因子和维生素的代谢 metabolism of cofactors and vitamins 1
能量代谢 energy metabolism 1
氨基酸代谢 amino acid metabolism 1
转录 transcription 2
翻译 translation 1
折叠, 分选和降解 folding, sorting and degradation 1
信号转导 signal transduction 12
细胞群落型真核生物 cellular-community eukaryotes 3 5
转导和代谢 transport and catabolism
KEGG 通路 KEGG pathway 细胞生长和死亡 cell growth and death 1 3 8
细胞迁移 cell motility
内分泌系统 endocrine system
神经系统 nervous system
免疫系统 immune system 2 2
环境适应力 environmental adaptation 1
循环系统 circulatory adaptation 1
衰老 aging 1
感染性疾病：病毒 infectious disease: viral 6
癌症：概览 cancer: overview 6
癌症：具体类型 cancer: specific types 4
神经退行性疾病 neurodegenerative disease 3
物质依赖 substance dependence 2
感染性疾病：寄生虫 infectious disease: parasitic 1
免疫疾病 immune disease 1
内分泌和代谢疾病 endocrine and metabolic disease 1
药物抵抗：抗肿瘤药物 drug resistance: antineoplastic 1
心血管疾病 cardiovascular disease 1
0 5 10
基因数量/个
no. of genes
(c)
图 7 与 EseJ 互作的宿主蛋白鉴定及 KEGG 通路分析
(a) 基于小鼠蛋白库筛选潜在与 EseJ 互作的宿主蛋白。散点图是根据 eseJ-HA 组中唯一肽段数与 HA 组中唯一肽段数的比值绘制，圆圈中
的点表示 eseJ-HA 组中特异性存在的 103 个蛋白。由于计算得出的坐标位置相同，这些蛋白重叠在同一点。根据离原点的距离，每个点分
别代表 95 个、6 个和 2 个蛋白。箭头指向 NDUFA7。(b) 细菌双杂交揭示 EseJ 和 NDUFA7 的直接互作。(c) KEGG 通路分析揭示潜在与
EseJ 互作的宿主蛋白所在的细胞通路。
Fig. 7 Identification of proteins interacting with EseJ and KEGG pathway analysis
(a) the scatter plot illustrates the abundance of proteins across different groups. The scatter plot is constructed based on the ratio of the number of unique
peptides in the eseJ-HA group to those in the HA group， the points within the circle represent 103 proteins specifically present in the eseJ-HA group.
Due to identical calculated coordinate positions, these proteins overlap at the same point. Based on the distance from the origin, each point corresponds to
95, 6, and 2 proteins, respectively. The arrow indicates NDUFA7. (b) bacterial two hybrid assay validated the interaction between EseJ and NDUFA7. (c)
KEGG pathway analysis was performed for the 103 candidate proteins interacting with EseJ.

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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