Page 193 - 《水产学报》2025年第8期

曹磊，等 水产学报, 2025, 49(8): 089416

ACACA 活性可以抑制人类和小鼠中 TH17 细胞 networks[J]. Trends in Microbiology, 2011, 19(6): 268-277.
的形成，并促进抗炎的 Foxp3 调节性 T (Treg) [ 6 ] Mitosch K, Beyß M, Phapale P, et al. A pathogen-specific iso-
+
细胞的发展，从而建立抑制炎症应答的环境 。 tope tracing approach reveals metabolic activities and fluxes of
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根据这一结论，本实验推测，杀鱼爱德华氏菌 intracellular Salmonella[J]. PLoS Biology, 2023, 21(8):
在感染 J774A.1 细胞时分泌 T3SS 效应蛋白 EseJ， e3002198.
其与 ACACA 结合抑制其活性，抑制宿主炎症 [ 7 ] Takaya A, Yamamoto T, Tokoyoda K. Humoral immunity vs.
反应以促进杀鱼爱德华氏菌定殖，而 PPARγ 的 Salmonella[J]. Frontiers in Immunology, 2020, 10: 3155.
上调则可以归因于 Acaca 活性抑制所带来的负 [ 8 ] Leung K Y, Wang Q Y, Yang Z Y, et al. Edwardsiella pisci-
反 馈 效 应 。 总 之 ， 本 研 究 中 对 感 染 过 程 中 cida: a versatile emerging pathogen of fish[J]. Virulence, 2019,
T3SS 效应蛋白 EseJ 功能进行了初步解析，还 10(1): 555-567.
需对其操控 PPARγ 蛋白丰度促进病原菌感染定 [ 9 ] Park S B, Aoki T, Jung T S. Pathogenesis of and strategies for
殖的机制进一步探索。这将有助于更好地理解 preventing Edwardsiella tarda infection in fish[J]. Veterinary
杀鱼爱德华氏菌的致病机制，并为进一步提出 Research, 2012, 43(1): 67.
更具针对性的控制措施提供理论依据。 [10] Buján N, Toranzo A E, Magariños B. Edwardsiella piscicida: a

significant bacterial pathogen of cultured fish[J]. Diseases of
4 结论 Aquatic Organisms, 2018, 131(1): 59-71.
[11] Xie H X, Yu H B, Zheng J, et al. EseG, an effector of the type
杀鱼爱德华氏菌作为一种重要的水产病原
III secretion system of Edwardsiella tarda, triggers microtu-
菌，其致病机制主要依赖于 T3/T6SS 及其效应
bule destabilization[J]. Infection and Immunity, 2010, 78(12):
蛋白。本研究基于在杀鱼爱德华氏菌感染巨噬
5011-5021.
细胞模型，发现杀鱼爱德华氏菌利用效应蛋白
[12] Cao H F, Han F J, Tan J C, et al. Edwardsiella piscicida type
EseJ 增加 PPARγ 蛋白丰度。受 EseJ 诱导产生
III secretion system effector EseK inhibits mitogen-activated
的 PPARγ 有效抑制促炎性细胞因子表达，缓解 protein kinase phosphorylation and promotes bacterial coloniza-
线粒体损伤，削弱胞内 ROS 产生，促进杀鱼爱 tion in zebrafish larvae[J]. Infection and Immunity, 2018, 86(9):
德华氏菌在巨噬细胞内增殖。因此，本研究将 e00233-18.
加 深 对 杀 鱼 爱 德 华 氏 菌 T3SS 效 应 蛋 白 EseJ [13] Hou M Y, Chen R, Yang D H, et al. Identification and func-
在其感染致病机制中作用的理解，为针对杀鱼 tional characterization of EseH, a new effector of the type III
爱德华氏菌感染的防控提供理论基础和候选 secretion system of Edwardsiella piscicida[J]. Cellular Microbi-
靶标。 ology, 2017, 19(1): e12638.
[14] He T T, Zhou Y, Liu Y L, et al. Edwardsiella piscicida type III
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