Page 158 - 《水产学报》2025年第8期

王一凡，等 水产学报, 2025, 49(8): 089613

Regulation of different fatty acids on carbohydrate response element
binding protein (ChREBP) in Larimichthys crocea

WANG Yifan , ZHAO Zengqi , CHEN Fan , SHEN Yanan ,
XU Wei , MAI Kangsen , AI Qinghui *
Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Key Laboratory of Mariculture,
Ministry of Education, Fisheries College, Ocean University of China, Qingdao 266003, China

Abstract: Carbohydrate response element binding protein (ChREBP) is a key transcription factor in the regulation of glucose
and lipid metabolism in mammals. However, the function of ChREBP and its response to fatty acids in fish are still unknown.
Therefore, in this study, we chose the large yellow croaker (Larimichthys crocea) as the research object, and firstly, this study
cloned the sequence of the CDS region of the ChREBP gene in L. crocea. The results found that the full length of the CDS
sequence of ChREBP in L. crocea is 2 808 bp, encoding 935 amino acids. Considering that the sequence of ChREBP has a cer-
tain degree of homology compared with the sequence of the mammal, ChREBP may have conserved functions in L. crocea.
Real-time fluorescence quantitative PCR (RT-qPCR) was used to analyze the expression pattern of ChREBP gene in different
tissues of L. crocea, and the results found that the mRNA levels of ChREBP gene were highest in the intestine of L. crocea,
while it was also highly expressed in the liver. After 10 weeks of feeding trial, the mRNA levels of ChREBP gene were signi-
ficantly lower (P < 0.05) in the liver of L. crocea fed palm oil, olive oil, linseed oil, and soya bean oil, compared with fish fed
fish oil. After incubation of fish hepatocytes with different fatty acids for 12 h, the mRNA levels of ChREBP gene were signi-
ficantly (P < 0.05) reduced in the palmitic acid (PA, C16:0) treatment, compared with the control treatment. However, the
mRNA levels of ChREBP gene in linoleic acid (LA, C18:2n-6), linolenic acid (ALA, C18:3n-3), eicosapentaenoic acid (EPA,
C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3) treatments also showed a decreasing trend, but there was no significant
difference (P > 0.05). The above results indicate that ChREBP of L. crocea could respond to the stimulation of different fatty
acids at both in vivo and in vitro levels and might play an important role in the regulation of hepatic glucose and lipid metabol-
ism. The above studies have further deepened the understanding of the mechanism of glucose and lipid metabolism regulation
in fish, and provided a theoretical basis for improving the glucose and lipid metabolism disorders induced by the substitution of
vegetable oils in L. crocea.

Key words: Larimichthys crocea; glucose metabolism and lipid metabolism; fatty acid; gene expression; carbohydrate response
element binding protein (ChREBP)
Corresponding author: AI Qinghui. E-mail: qhai@ouc.edu.cn
Funding projects: China Agriculture Research System (CARS-47-11); National Natural Science Foundation of China
(31830103)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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