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车行，等 水产学报, 2025, 49(5): 059114

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Bioinformatic characteristics, excision/cyclization activities and

prevalence of an ICE element, ICESag1535, in
Streptococcus agalactiae isolated from fish

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CHE Hang , LU Yanbo , WANG Chengde , LUO Minyi , LI Guo ,
1
4
ZHANG Defeng , LIN Li , ZHAO Lijuan 1*
1. College of Animal Science and Technology,
Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
2. Agricultural Service Center of Xiaolan Town, Zhongshan 510230, China;
3. Bailian Aquatic Seedling Co., Ltd. of Gaozhou City, Maoming 525200, China;
4. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

Abstract: With the exponential expansion of sequenced complete streptococcal genomes, numerous integrative and conjugat-
ive elements (ICEs) have been identified. However, the characteristics of ICEs in Streptococci spp. of aquatic animal origin
remain enigmatic. This study aims to analyze the bioinformatic characteristics of ICEs originating from aquatic animals, verify
their horizontal transfer capabilities, investigate the prevalence in bacteria, predict their microbial host distribution for hori-
zontal diffusion, and detect their contributions to pathogenicity of host bacteria. Integrated genomic approaches were employed
to systematically characterize the structural-functional attributes and epidemiological distribution of ICEs, complemented by in
vitro functional assays to validate their horizontal gene transfer capacity and molecular mechanisms. A complete ICE element

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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