Page 66 - 《渔业研究》2025年第6期

第 6 期 金楠霖等： 红尾皇冠鱼源彭氏变形杆菌的分离、鉴定及药敏实验 757

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Isolation, identification and antimicrobial susceptibility testing of
Proteus penneri from Aequidens rivulatus

JIN Nanlin，SHENG Dong，HAO Kexin，ZHANG Yu，REN Xu，YANG Jiahao，YE Shigen *
(Aquatic Animal Hospital, Dalian Ocean University, Dalian 116023, China)

Abstract: [Objective] This study aimed to conduct isolation, identification, and antimicrobial susceptibility
testing of pathogenic bacteria from diseased red-tailed crown fish (Aequidens rivulatus), providing theoretical
basis and practical guidance for the prevention and control of diseases in A. rivulatus. [Methods] The strain
was identified through morphological observation, physiological and biochemical characteristic analysis, 16S
rRNA gene sequencing, and phylogenetic tree construction. Its susceptibility to antimicrobial agents was also
determined. [Results] The results showed that the physiological and biochemical characteristics of the isolated
bacterium were consistent with those of Proteus penneri. Meanwhile, molecular identification results and phylo-
genetic tree analysis indicated that the strain had a close genetic relationship with P. penneri, thus confirming
the isolated pathogen as P. penneri. In the regression infection test, the isolated strain was found to be pathogen-
ic to A. rivulatus. For individual A. rivulatus weighing approximately 50 g, the median lethal dose (LD ) was
50
5
5.16×10 CFU/ind. Antimicrobial susceptibility testing showed that the strain was sensitive to ciprofloxacin, en-
rofloxacin, and trimethoprim-sulfamethoxazole, but resistant to penicillin and ceftriaxone. [Conclusion] In
summary, the study confirms that P. penneri is the pathogenic bacterium causing diseases in A. rivulatus. On the
basis of ensuring the safety of aquatic drug use, enrofloxacin and ciprofloxacin can be used as effective drugs
for treating infections in A. rivulatus.
Key words: Aequidens rivulatus; Proteus penneri; bacterial isolation and identification; pathogenicity;
antimicrobial susceptibility test