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第 3 期       吴隐生等: 基于      RAA-LbCas12a  技术的有毒(害)赤潮快速检测产品应用效果评估                         303

                   algal blooms on human health: a systematic review[J].  strip for rapid detection of Gymnodinium catenatum[J].
                   Journal of Water and Health, 2019, 17(4): 499 − 516.  Environmental Monitoring and Assessment, 2023, 195(1):
              [  6  ]   中华人民共和国中央人民政府. 福建:耕海牧渔 建设                   83.

                   “海上粮仓”[EB/OL]. (2024-05-21)[2025-01-25].   [14]   Li H R, Meng K, Yu Q J, et al. Rapid detection method
                   https://www.gov.cn/yaowen/tupian/202405/content_  of  Skeletonema  pseudocostatum  and  preparation  of  test
                   6952639.htm#1.                                   strip[J]. Environmental Science and Pollution Research,
                   The  State  Council  of  the  People’s  Republic  of  China.  2022, 29(46): 70202 − 70208.
                   Fujian: harnessing marine resources and advancing fish-  [15]   Wang L, Zhuang Y Y, Zhang H, et al. DNA barcoding
                   eries to establish a “granary on the sea”[EB/OL]. (2024-  species  in  Alexandrium  tamarense  complex  using  ITS
                   05-21)[2025-01-25]. https://www.gov.cn/yaowen/tupian/  and  proposing  designation  of  five  species[J].  Harmful
                   202405/content_6952639.htm#1.                    Algae, 2014, 31: 100 − 113.
              [  7  ]   何恩业,季轩梁,李晓,等. 2001—2020   年福建沿海       [16]   郑俊斌,张凤英,马凌波,等. 米氏凯伦藻               18S
                   赤潮灾害分级和时空分布特征研究             [J]. 海洋通报,           rDNA  和转录间隔区序列分析       [J]. 上海海洋大学学
                   2021,40(5) :578 − 590.                           报,2009,18(6) :680 − 685.
                   He E Y, Ji X L, Li X, et al. The spatial-temporal distri-  Zheng J B, Zhang F Y, Ma L B, et al. Sequence analysis
                   bution  and  hazard  grading  of  red  tides  in  Fujian  coast  of Karenia mikimotoi based on ribosomal 18S rDNA and
                   waters during 2001—2020[J]. Marine Science Bulletin,  internal transcribed space regions[J]. Journal of Shang-
                   2021, 40(5): 578 − 590.                          hai Ocean University, 2009, 18(6): 680 − 685.
              [  8  ]   毛迎雪,刘蒙达,张皓博,等. 重组酶介导等温扩增              [17]   王建艳,何建宗,齐雨藻,等. 甲藻(Dinophyta)凯
                   技术(RAA)在病原微生物检测中的应用进展                [J].        伦藻科(Kareniaceae)的分类学研究与展望         [J]. 海
                   中国动物检疫,2024,41(1) :60 − 66.                      洋与湖沼,2017,48(4) :786 − 797.
                   Mao Y X, Liu M D, Zhang H B, et al. Advances on ap-  Wang J Y, He J Z, Qi Y Z, et al. Progress in taxonomy
                   plication of  recombinase  aided  amplification  in   detec-  study  on  Kareniaceae  (Dinophyta)[J].  Oceanologia  et
                   tion  of  pathogenic  microorganisms[J].  China  Animal  Limnologia Sinica, 2017, 48(4): 786 − 797.
                   Health Inspection, 2024, 41(1): 60 − 66.   [18]   吕颂辉,岑竞仪,王建艳,等. 我国近海米氏凯伦藻
              [  9  ]   Yuan X H, Yuan H, Liu B X, et al. Self-supplying guide  (Karenia mikimotoi)藻华发生概况、危害及其生态
                   RNA-mediated CRISPR/Cas12a fluorescence system for  学机制  [J]. 海洋与湖沼,2019,50(3) :487 − 494.
                   sensitive  detection  of  T4  PNKP[J].  Molecules,  2022,  Lü S H, Cen J Y, Wang J Y, et al. The research status
                   27(24): 9019.                                    quo,  hazard,  and  ecological  mechanisms  of  Karenia
              [10]   Wang L, Chen X Y, Pan F F, et al. Development of a  mikimotoi red tide in coastal waters of China[J]. Ocean-
                   rapid detection method for Karenia mikimotoi by using  ologia et Limnologia Sinica, 2019, 50(3): 487 − 494.
                   CRISPR-Cas12a[J].  Frontiers  in  Microbiology,  2023,  [19]   Heil  C  A,  Bronk  D  A,  Dixon  L  K,  et  al.  The  gulf  of
                   14: 1205765.                                     Mexico  ECOHAB:  Karenia  program  2006 —2012[J].
              [11]   Wu Y S, Chen E H, Sun B S, et al. Establishment and  Harmful Algae, 2014, 38: 3 − 7.
                   application of recombinase-aided amplification combined  [20]   Cai  P  J,  He  S,  Zhou  C  X,  et  al.  Two  new  karlotoxins
                   with LbCas12a for the rapid detection of Karenia brevis  found in Karlodinium veneficum (strain GM2) from the
                   [J/OL].  (2024-11-12)[2025-01-05].  https://doi.org/10.  East China Sea[J]. Harmful Algae, 2016, 58: 66 − 73.
                   21203/rs.3.rs-5300931/v1.                  [21]   徐娜,逄少军,刘峰. 一株桑沟湾赤潮藻的分子鉴
              [12]   Fang J H, Liu X X, He W X, et al. Establishment of col-  定  [J]. 海洋科学,2012,36(4) :13 − 18.
                   loidal gold immunochromatography strip for rapid detec-  Xu  N,  Pang  S  J,  Liu  F.  Molecular  identification  of  a
                   tion of Karen mikimotoi and its application[J]. Environ-  bloom-forming  species  isolated  from  Sanggou  Bay  in
                   mental  Science  and  Pollution  Research,  2023,  30(50):  Shandong  Province[J].  Marine  Sciences,  2012,  36(4):
                   109123 − 109134.                                 13 − 18.
              [13]   Fang J H, Li S Y, Liu X X, et al. Development of a test  [22]   Dai X F, Lu D D, Guan W B, et al. Newly recorded Kar-
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