Page 39 - 《水产学报》2026年第2期

2 期 李 双，等：跨组织/性别系统评估曼氏无针乌贼内参基因的稳定性 50 卷

male
3.0
brain 0.273 0.105 0.295 1.557 0.886
optic lobe 0.733 0.329 0.204 1.017 0.236
heart 1.226 0.129 0.250 0.064 0.441
gill 0.773 0.074 0.074 0.810 0.533
branchial heart 0.038 0.038 0.065 0.677 0.664
intestine 0.375 0.522 0.336 1.944 1.362
stomach 0.584 0.423 0.198 1.331 2.508
caecus 0.775 0.278 0.090 1.004 0.474
1.5
kidney 2.302 0.323 0.304 0.452 1.004
liver 0.659 0.401 0.133 0.277 0.421
pancreas 0.426 1.314 0.110 0.623 0.184
white body 0.354 0.634 0.353 1.408 0.529
skin 0.873 0.849 0.108 1.059 0.401
testis 0.449 0.559 0.179 0.797 2.032
spermatophore 0.872 0.471 0.124 0.153 0.818
all tissues 0.952 0.160 0.503 2.901 1.515
0
18S ef-1α ef-1γ β-actin gapdh
(a)
female
3.0
brain 0.356 0.625 0.523 0.499 1.590
optic lobe 0.652 0.253 0.723 0.952 0.479
heart 0.172 0.096 0.587 0.644 0.432
gill 1.643 0.304 0.738 1.824 1.265
branchial heart 0.442 0.308 0.206 0.620 0.758
intestine 0.136 0.409 0.136 0.863 0.802
stomach 0.118 0.164 0.051 0.450 0.685
caecus 2.169 0.218 2.417 0.798 2.091
kidney 0.723 0.165 0.377 1.069 1.127 1.5
liver 0.710 0.745 0.427 0.981 1.746
pancreas 0.352 0.111 0.227 0.426 0.439
white body 0.295 0.310 0.287 1.190 0.584
skin 0.069 0.235 0.532 1.038 0.069
ovary 0.199 0.978 0.199 1.516 2.209
nidamental gland 0.785 1.052 1.334 0.426 1.556
accessory nidamental gland 0.483 0.281 0.416 2.028 0.733
all tissues 1.028 0.348 1.253 2.998 1.721
0
18S ef-1α ef-1γ β-actin gapdh
(b)
Fig. 5 Expression stability and ranking of reference genes as determined by NormFinder
The stability values of each gene in different tissues are exhibited. Lower values (blue) indicate higher stability, higher values (red) indicate lower
stability.

of the tissues examined, specifically the optic lobe, only acceptable gene in the pancreas. These findings
heart, branchial heart, intestine, stomach, pancreas, suggested a more favorable performance of ef-1α and
white body, and skin. Furthermore, the results showed ef-1γ compared to 18S within female S. japonica, a
that ef-1α and ef-1γ exhibited an SD < 1 in fifteen and finding that differs from the results obtained for male
fourteen tissues, respectively; 18S in twelve, gapdh in S. japonica. No suitable gene was found in the nida-
eleven, and β-actin in nine. Besides, ef-1α was the mental gland, even though β-actin (SD = 1.07) exhib-

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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