Page 37 - 《水产学报》2026年第2期

2 期 李 双，等：跨组织/性别系统评估曼氏无针乌贼内参基因的稳定性 50 卷

1.5 brain 1.0 0.849 optic lobe 0.8 heart
M value of geNorm 1.0 1.026 0.612 0.236 0.183 0.180 M value of geNorm 0.8 0.700 0.530 0.458 0.440 M value of geNorm 0.6 0.606 0.524 0.460 0.411 0.400

0.6
0.4
0.4
0.5
0.2
0.2
0
4
2
2
5
4
5
1
2
4
5
different genes 3 0 1 different genes 3 0 1 different genes 3
(a) branchial heart intestine
(c)
(b)
M value of geNorm 1.5 1.681 1.388 0.686 0.555 0.540 M value of geNorm 0.8 0.687 0.571 0.457 0.270 0.260 M value of geNorm 0.8 0.724 0.574 0.382 0.273 0.250
gill
2.0
0.6
0.6
0.4
1.0
0.4
0.2
0.2
0.5
0
2
1
2
5
4
3
4
1
3
4
different genes 5 0 2 different genes 5 0 1 different genes 3
(f)
(e)
(d) 3.0 caecus 1.5 kidney
M value of geNorm 0.4 0.469 0.310 0.237 0.226 0.200 M value of geNorm 2.0 2.386 2.144 1.922 0.975 0.850 M value of geNorm 1.0 1.009 0.867 0.731 0.329 0.310
stomach
0.6
1.0
0.5
0.2
0
3
3
1
2
3
4
1
1
4
2
different genes 5 0 4 different genes 5 0 2 different genes 5
(h)
(g) 0.6 pancreas 1.0 white body
(i)
M value of geNorm 1.0 1.350 0.994 0.773 0.305 0.300 M value of geNorm 0.4 0.449 0.396 0.269 0.222 0.210 M value of geNorm 0.8 0.821 0.538 0.467 0.304 0.290
liver
1.5
0.6
0.4
0.5
0.2
0.2
0
1
3
4
4
3
4
2
5
2
1
different genes 5 0 2 different genes 5 0 1 different genes 3
(l)
(k)
(j) 1.8 ovary 1.8 nidamental gland
M value of geNorm 0.6 0.605 0.308 0.230 0.138 0.110 M value of geNorm 1.2 1.578 1.063 0.533 0.397 0.380 M value of geNorm 1.2 1.439 1.256 1.131 0.257 0.250
skin
0.8
0.4
0.6
0.6
0.2
0
4
2
5
1
5
1
4
3
1
4
different genes 3 0 2 different genes 3 0 2 different genes 5
(m) 1.5 accessory nidamental gland (n) 2.5 2.276 all tissues (o)
M value of geNorm 1.0 1.220 0.644 0.363 0.242 0.241 M value of geNorm 2.0 1.649 1.407 0.914 0.905
1.5
1.0
0.5
0.5
0
3
1
2
3
4
4
2
different genes 5 0 1 different genes 5
(p) (q)
Fig. 4 geNorm assessment of reference gene expression stabilities and rankings in female S. japonica
Five candidate genes were ranked in different tissues as described in Fig. 3. The orange column indicates gene with a M value greater than the cut-off
value of 1.5.
中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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