Page 40 - 《水产学报》2026年第2期

2 期 水 产 学 报 50 卷

male
brain 0.35 0.19 0.21 1.06 0.47
optic lobe 0.39 0.57 0.34 1.03 0.40
heart 0.85 0.18 0.27 0.30 0.33
gill 0.40 0.84 0.79 1.04 0.86
coefficient of variation
branchial heart 0.29 0.23 0.36 0.50 0.55
1.00
intestine 0.36 0.74 0.75 1.04 1.01 3.72
stomach 0.88 1.50 1.32 1.48 1.80 13.81
caecus 0.64 1.15 1.07 1.26 1.42
standard deviation
kidney 0.76 2.40 1.99 2.35 2.63 3
liver 0.60 0.94 0.79 0.89 0.85
pancreas 0.29 1.05 0.27 0.19 0.51
2
white body 0.50 1.00 0.80 0.48 0.87
skin 1.17 2.08 1.76 1.21 1.86
1
testis 0.85 1.49 1.23 1.58 1.01
spermatophore 1.04 1.20 1.12 0.81 0.98
all tissues 1.06 1.44 1.25 2.76 1.88 0
18S ef-1α ef-1γ β-actin gapdh
(a)
female
brain 0.29 0.39 0.38 0.78 1.59
optic lobe 0.69 0.34 0.38 0.45 0.59
heart 0.23 0.12 0.42 0.41 0.28
gill 1.03 0.79 0.57 2.09 1.25
coefficient of variation
branchial heart 0.38 0.46 0.40 0.58 0.23
0.65
intestine 0.85 0.76 0.74 0.57 0.84 2.96
stomach 0.34 0.31 0.42 0.47 0.46 13.46
caecus 1.57 0.90 1.34 1.34 1.53
kidney 0.77 0.43 0.57 1.04 0.39 standard deviation
3
liver 1.01 1.16 0.99 1.06 0.87
pancreas 0.39 0.22 0.28 0.36 0.45
white body 0.35 0.20 0.36 0.75 0.38 2
skin 0.22 0.17 0.26 0.76 0.17
ovary 0.33 0.85 0.46 1.07 1.20
1
nidamental gland 1.23 1.44 1.46 1.07 1.39
accessory nidamental gland 0.60 0.40 0.48 1.26 0.72
all tissues 1.42 0.98 1.16 2.65 1.50 0

18S ef-1α ef-1γ β-actin gapdh
(b)
Fig. 6 Reference genes stability evaluated by BestKeeper
Two parameters, the standard deviation (SD) values and the standard coefficient of variation (CV) values, are marked by colors and the circle size,
respectively. The values displayed are SD values. Lower values (blue) and smaller circle indicate higher stability, higher values (red) and larger circle
indicate lower stability.
ited the closest SD value to the 1.0 threshold. Consist- results.
ently, gapdh and β-actin occupied the lowest stability
2.6 ΔC analysis
t
rankings. The pooled analysis across all tissues
revealed a rank order as follows: ef-1α > ef-1γ > 18S > The ΔC approach was additionally employed to
t
gapdh > β-actin, thereby supporting the overall pat- assess the expression stability of reference genes by
tern observed in the tissue-specific quantification calculating the standard deviation (SD) of ΔC values
t
https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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