Page 34 - 《水产学报》2026年第2期

2 期 水 产 学 报 50 卷

30 male 30 female
25 bc 25
C t value 20 abc a ab a abc c a a a C t value 20 abcdef abcg abcd cdef ef f def bcdef

15 a a a a a 15 abcde ag g abcdg abg abcg g abcdg
10 10
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10111213161718
tissue tissue
(a) (b)
30 f 30 f
bcde f de ef
25 abcd cdef abcde 25 a a ad ad a ad ad ad ad ed
C t value 20 a abc ab a abc abc C t value 20 bce ce ed

15 g 15 bc b
10 10
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10111213161718
tissue tissue
(c) (d)
30 d 30
abc
25 abcd abc abcd abcd cd bcd 25 d bcd d d d cd cd
C t value 20 a ab a abc a abc a C t value 20 abc abc abc abcd cd ab a abc bcd

15 15
10 10
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10111213161718
tissue tissue
(e) (f)
30 30
ab ab
25 ab b ab ab 25 e de
C t value 20 a a a ab a a ab a a C t value 20 abcd abcd abc abc abcd abcd ab a bcde a ab
cde
abcd abcde
15 15
10 10
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10111213161718
tissue tissue
(g) (h)
30 30
abc bc
25 ab abc abc c ab abc abc 25 abcde cde de f ef
C t value 20 ab a ab ab ab ab C t value 20 abcde ef cde bcde cde bcde a ef abcd ab
abc
15
15
10 10
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10111213161718
tissue tissue
(i) (j)
18S β-actin gapdh ef-1α ef-1γ
Fig. 1 Tissue-specific C t profiles of five reference genes in male and female S. japonica
(a) (c) (e) (g) (i) male, (b) (d) (f) (h) (j) female. Medians are indicated by lines across the box. Whisker caps denote the maximum and minimum values.
Different colors indicate different candidate genes, n = 5. Statistical significance between male and female groups was performed using One-Way
ANOVA followed by Tukey’s multiple comparison test. Statistical significances were accepted at P < 0.05 or greater. Different letters indicate statistic-
ally significance. The same below. 1. brain, 2. optic lobe, 3. heart, 4. gill, 5. branchial heart, 6. intestine, 7. stomach, 8. caecus, 9. kidney, 10. liver, 11.
pancreas, 12. white body, 13. skin, 14. testis, 15. spermatophore, 16. ovary, 17. nidamental gland, 18. accessory nidamental gland.

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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