Page 223 - 《水产学报》2026年第01期

1 期 李中林，等：基于胶体金免疫层析技术快速检测鱼糜中的卵白蛋白 50 卷

5%~10%，甚至更高 。因此，过于追求较低的检 47(11): 119817 (in Chinese).
[8]
测限对于鱼糜制品中 OVA 的检测实际应用意义不 [ 8 ] 王卉楠, 励建荣, 李学鹏, 等. 鱼糜组分间相互作用对其凝胶
特性影响的研究进展 [J]. 中国食品学报, 2022, 22(9): 365-375.
大。由于鸡 OVA 和鸭 OVA 的氨基酸序列同源度较
Wang H N, Li J R, Li X P, et al. Research progress on the effect
高 [35] ，所开发的检测试纸对二者均有特异性反
of component interactions on the gel properties of surimi[J].
应，达到了对鱼糜中鸡蛋清 OVA、鸭 OVA 的检
Journal of Chinese Institute of Food Science and Technology,
测目标要求。接下来可从优化检测抗体的角度出 2022, 22(9): 365-375 (in Chinese).
发，制备单克隆抗体、纳米抗体等，有针对性地 [ 9 ] 赵勇娟, 翁凌, 颜龙杰, 等. 竞争性 ELISA 检测鱼糜制品中蛋
区分两种不同禽类的 OVA。此外，应进一步改进 清含量 [J]. 食品科学, 2017, 38(14): 284-289.
检测试纸的性能，为其他复杂食品基质中 OVA 的 Zhao Y J, Weng L, Yan L J, et al. Quantification of added egg
快速灵敏检测提供理论和技术支持。 white in surimi products by competitive enzyme linked-immun-
osorbent assay[J]. Food Science, 2017, 38(14): 284-289 (in
总之，本研究开发的检测试纸可以实现现场、
Chinese).
快速检测鱼糜原料及制品中的 OVA，配合胶体金
[10] 李钰金, 林洪, 赵元晖, 等. 蛋白质类添加物在鱼糜制品中的
试纸分析仪可实现对 OVA 的定量检测。整个检测 应用现状及发展趋势 [J]. 食品研究与开发, 2020, 41(5): 220-
方法特异性好、成本低、速度快，较其他现有的 224.
检测方法具有一定优势。 Li Y J, Lin H, Zhao Y H, et al. Application status and develop-
ment trend of protein additives in fish surimi products[J]. Food
（作者声明本文无利益冲突） Research and Development, 2020, 41(5): 220-224 (in Chinese).
[11] Huang Y H, Li R R, Zhu W Y, et al. Development of a fluores-
参考文献 (References)： cent multiplexed lateral flow immunoassay for the simultan-
eous detection of crustacean allergen tropomyosin, sarcoplas-
[ 1 ] Ma B, Fu X, Zhu P, et al. Allergenicity, assembly and applica-
mic calcium binding protein and egg allergen ovalbumin in dif-
tions of ovalbumin in egg white: a review[J]. Critical Reviews
ferent matrices and commercial foods[J]. Food Chemistry,
in Food Sceince and Nutrition, 2024, 64(24): 8672-8688.
2024, 440: 138275.
[ 2 ] Rostamabadi H, Chaudhary V, Chhikara N, et al. Ovalbumin,
[12] Mattarozzi M, Milioli M, Bignardi C, et al. Investigation of dif-
an outstanding food hydrocolloid: applications, technofunc-
ferent sample pre-treatment routes for liquid
tional attributes, and nutritional facts, a systematic review[J].
chromatography–tandem mass spectrometry detection of case-
Food Hydrocolloids, 2023, 139: 108514.
[ 3 ] Liu E G, Tan J, Munoz J S, et al. Food matrix composition ins and ovalbumin in fortified red wine[J]. Food Control, 2014,
38: 82-87.
affects the allergenicity of baked egg products[J]. The Journal
of Allergy and Clinical Immunology: In Practice, 2024, 12(8): [13] Baldo T A, Proença C D A, da Silva Felix F, et al. Disposable
2111-2117. electrochemical microfluidic device for ultrasensitive detection
[ 4 ] Wang Z L, Zhang J, Yuan J, et al. Oral administration of egg of egg allergen in wine samples[J]. Talanta, 2021, 232: 122447.
ovalbumin allergen induces dysregulation of tryptophan meta- [14] Azarnia S, Boye J I, Mongeon V, et al. Detection of ovalbumin
bolism in sensitized BALB/c mice[J]. Food & Function, 2024, in egg white, whole egg and incurred pasta using LC-ESI-
15(8): 4375-4388. MS/MS and ELISA[J]. Food Research International, 2013,
[ 5 ] Shifti D M, Soriano V X, Perrett K P, et al. Milk and egg 52(2): 526-534.
allergy are associated with increased risk of peanut allergy at [15] 龚频, 王思远, 陈雪峰, 等. 胶体金免疫层析试纸条技术及其
age 1 in Australia[J]. The Journal of Allergy and Clinical 在食品安全检测中的应用研究进展 [J]. 食品工业科技, 2019,
Immunology: In Practice, 2024, 12(10): 2868-2869. e2. 40(13): 358-364.
[ 6 ] Csurka T, Varga-Tóth A, Kühn D, et al. Comparison of techno- Gong P, Wang S Y, Chen X F, et al. Research progress of col-
functional and sensory properties of sponge cakes made with loidal gold immunochromatographic test strip technology and
egg powder and different quality of powdered blood products its application in food safety testing[J]. Science and Techno-
for substituting egg allergen and developing functional food[J]. logy of Food Industry, 2019, 40(13): 358-364 (in Chinese).
Frontiers in Nutrition, 2022, 9: 979594. [16] Wang Y D, Fu Y W, Miao K, et al. A colloidal gold immuno-
[ 7 ] 薛长湖, 李兆杰. 我国水产品加工和流通产业科技现状与发 chromatographic method for rapid screening of imidacloprid
展趋势 [J]. 水产学报, 2023, 47(11): 119817. residues in Chinese herbal medicines[J]. Journal of Chromato-
Xue C H, Li Z J. Current situation and development trend of graphy B, 2024, 1244: 124240.
science and technology in aquatic product processing and circu- [17] 徐超莲, 赖卫华, 刘道峰. 胶体金免疫层析法检测食品中天然
lation industry of China[J]. Journal of Fisheries of China, 2023, 存在的危害物质的研究进展 [J]. 食品科学, 2014, 35(5): 257-261.

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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