Page 225 - 《水产学报》2026年第01期

1 期 李中林，等：基于胶体金免疫层析技术快速检测鱼糜中的卵白蛋白 50 卷

Rapid detection of ovalbumin in surimi based on colloidal gold
immunochromatography assay

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LI Zhonglin , HUANG Jianlian 2, 3* , ZHANG Lingjing , ZHANG Changgong , CHEN Yulei ,
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WENG Ling , CAO Minjie 1*
(1. College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China;
2. Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing in
Fujian Province, Xiamen 361028, China;
3. Anjoy Foods Group Co., Ltd., Xiamen 361028, China;
4. Xiamen Bonson Biotechnology Co., Ltd., Xiamen 361021, China)

Abstract: Egg white is widely used in the food industry due to its unique properties, particularly in the production and pro-
cessing of surimi products. However, some manufacturers excessively add egg white as a substitute for fish meat without
proper labeling, aiming to reduce production costs. Ovalbumin (OVA), a major allergen in egg white, is the primary protein of
concern. Currently, established standards for the use of OVA in surimi products are not available. The addition of non-fish pro-
teins, such as egg white protein, not only constitutes food adulteration but also causes significant health risks to individuals with
allergies due to the presence of OVA. Since there is no effective clinical treatment for food allergies, patients are advised to
avoid allergenic ingredients in their diets. Therefore, developing a rapid, simple, and sensitive detection method for allergens is
of practical significance. In this study, a rapid, simple, and on-site method for detecting the food allergen OVA in surimi raw
material and its products was developed based on the colloidal gold immunochromatographic assay (CGIA). Egg white was
pretreated using a two-phase aqueous extraction method, and OVA was purified by ion-exchange chromatography. The purity
of purified OVA was assessed via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and further con-
firmed by mass spectrometry. Polyclonal antibodies against OVA were prepared, and colloidal gold solution was synthesized
using the trisodium citrate reduction method. The antibodies were then labeled with colloidal gold. A colloidal gold immuno-
chromatographic test strip for OVA detection was developed based on a competitive assay principle and optimized. Western
blot analysis demonstrated that the anti-OVA polyclonal antibodies specifically recognized chicken egg OVA and duck egg
OVA without cross-reacting with other proteins in surimi, indicating high specificity. The developed OVA-CGIA-Strip could
rapidly detect chicken and duck OVAs in surimi. Four parameter logistic curve was used to fit the standard curve y=7.17-2.79
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lgx (R = 0.983 4). The linear range was 0.01 to 20.00 µg/mL, with a limit of detection (LOD) of 0.20 µg/mL and IC 50 of
(3.13±0.18) µg/mL. The OVA-CGIA method is simple to use, highly specific, and accurate. When combined with a colloidal
gold strip analyzer, it enables convenient, rapid, and quantitative detection of OVA in surimi. In summary, the OVA-CGIA-
Strip established in this study provides a simple, specific, and accurate approach for rapid on-site detection of OVA in surimi.
This method not only helps safeguard the health of allergic individuals but also ensures the integrity of surimi products, with
significant implications for both public health and the food industry. Its promising research and development prospects make it
a valuable tool for future applications.
Key words: ovalbumin; surimi; colloidal gold immunochromatographic assay; food allergen; rapid detection
Corresponding authors: HUANG Jianlian. E-mail: 416688254@qq.com;
CAO Minjie. E-mail: mjcao@jmu.edu.cn
Funding projects: National Key R & D Program of China (2018YFD0901004); Open Research Program of Fujian Key Labor-
atory of Refrigerated and Prepared Aquatic Products Processing (FJKLRCAPP2024-01)

中国水产学会主办 sponsored by China Society of Fisheries https://www.china-fishery.cn
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