Page 115 - 《水产学报》2025年第12期

程佩琳，等 水产学报, 2025, 49(12): 129309

Parentage analysis of offspring induced by artificial simulated spawning
environments in natural waters for natural breeding of
the Acipenser dabryanus

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CHENG Peilin , LIU Qingjuan , LI Pengcheng , WU Jinming , LI Junyi , LENG Xiaoqian ,
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WU Jinping , ZHONG Jia , YU Dan , LIU Huanzhang , DU Hao 1*
1. Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs,
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Abstract: In 2023, a successful natural breeding experiment for the Acipenser dabryanus was conducted in an artificial simu-
lated spawning environments within natural waters. To assess the effectiveness of this wild breeding experiment and to gain a
deeper understanding of the breeding and mating patterns of Acipenser dabryanus, this study employed the mitochondrial D-
loop region and 24 microsatellite for parentage assignment and genetic diversity analysis of parents and their offspring. The res-
ults showed that among the 20 parental sturgeons, 7 male and 7 female individuals participated in reproduction. Mitochondrial
DNA analysis indicated that 240 offspring shared two identical mitochondrial haplotypes with 20 parents. Furthermore,
microsatellite loci analysis revealed that these 240 offspring originated from 30 different parental combinations, with 223 off-
spring successfully matched to their parental lineage, resulting in a high matching success rate of 92.92%. Genetic diversity
analysis indicated that the 20 parental sturgeons exhibited a high level of genetic diversity, making them suitable as breeding
stock. The genetic diversity of the offspring group was essentially consistent with that of the parents, indicating that the wild
breeding experiment effectively maintained the genetic diversity to the offspring group. Three males contributed to 78.92% of
offspring, while four females accounted for 91.48% of the offspring. These results demonstrate that A. dabryanus adopts a
mixed mating strategy involving multiple females and males. This reproductive strategy is effective for the conservation genetic
diversity within the natural population of A. dabryanus. This study not only provides valuable information for the genetic
diversity and reproductive genetic management of A. dabryanus but also has significant guiding importance for future wild
release and population recovery efforts.
Key words: Acipenser dabryanus; parentage analysis; genetic diversity; mating pattern; population recovery
Corresponding authors: LIU Huanzhang. E-mail: hzliu@ihb.ac.cn;
DU Hao. E-mail: duhao@yfi.ac.cn
Funding projects: National Key Research and Development Program of China (2021YFD1200304); Central Public-interest
Scientific Institution Basal Research Fund, CAFS (2023TD08); National Natural Science Foundation of China (32303009)

https://www.china-fishery.cn 中国水产学会主办 sponsored by China Society of Fisheries
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